Hybridoma anti-DNA autoantibodies from patients with rheumatoid arthritis and systemic lupus erythematosus demonstrate similar nucleic acid binding characteristics.
Hybridoma anti-DNA autoantibodies from patients with rheumatoid arthritis and systemic lupus erythematosus demonstrate similar nucleic acid binding characteristics.
复制标题
来自类风湿性关节炎和系统性红斑狼疮患者的杂交瘤抗 DNA 自身抗体表现出相似的核酸结合特征。
作者：
J. Rauch;H. Massicotte;H. Tannenbaum
通过将GM 4672淋巴母细胞系与4名正常人、9名类风湿性关节炎（RA）患者和13名系统性红斑狼疮（SLE）患者的外周血淋巴细胞融合产生杂交瘤抗dna抗体。共获得441株杂交瘤克隆，其中37株分泌抗dna自身抗体。本文报道了2例正常人杂交瘤、9例RA患者杂交瘤和18例SLE患者杂交瘤产生的抗dna抗体的核酸结合特征。通过直接结合和竞争结合分析，三组杂交瘤抗DNA抗体对变性DNA （dDNA）、天然DNA （nDNA）、poly(I)、poly（dT）和cardiolipin均表现出相似的抗原结合特性。正常来源的抗dna抗体和自身免疫来源的抗体之间的一个区别是前者不能与z-DNA反应。然而，这需要更多正常克隆的进一步证实。在两种临床不同的自身免疫性疾病，即RA和SLE中发现的个体抗dna自身抗体对核酸抗原的反应性广泛重叠，这表明抗dna自身抗体的致病性可能与其核酸抗原结合特性无关。
Hybridoma anti-DNA antibodies have been generated from the fusion of the GM 4672 lymphoblastoid line with peripheral blood lymphocytes from four normal subjects, nine patients with rheumatoid arthritis (RA), and 13 patients with systemic lupus erythematosus (SLE). A total of 441 hybridoma clones were obtained, of which 37 secreted anti-DNA autoantibodies. The nucleic acid binding characteristics of the anti-DNA antibodies produced by two hybridomas from normal subjects, nine hybridomas from RA patients, and 18 hybridomas from SLE patients are reported. The hybridoma anti-DNA antibodies from all three groups showed similar antigen-binding characteristics for denatured DNA (dDNA), native DNA (nDNA), poly(I), poly(dT), and cardiolipin, by both direct binding and competitive binding analyses. One difference noted between normal-derived anti-DNA antibodies and autoimmune-derived antibodies was the inability of the former to react with z-DNA. However, this requires further substantiation with larger numbers of normal-derived clones. The broad overlap of reactivity to nucleic acid antigens among individual anti-DNA autoantibodies found in two clinically different autoimmune diseases, namely RA and SLE, suggests that the pathogenicity of anti-DNA autoantibodies may bear no relationship to their nucleic acid antigen-binding characteristics.