Transcriptome analysis and physical mapping of barley genes in wheat-barley chromosome addition lines
Transcriptome analysis and physical mapping of barley genes in wheat-barley chromosome addition lines
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DOI：
10.1534/genetics.105.049908
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发表时间：
2006-02-01
期刊：
影响因子：
3.3
通讯作者：
Muehlbauer, GJ
中科院分区：
文献类型：
--
作者：
Cho, SH;Garvin, DF;Muehlbauer, GJ
小麦-大麦染色体附加系是多种研究的有用遗传资源。本研究利用Barley1 Affymetrix基因芯片检测了Betzes大麦、中国春小麦和中国春-Betzes染色体附加系的转录本积累模式。在Betzes中检测到的4014个转录本中，在小麦-大麦二体染色体附加系2（2H）、3（3H）、4（4H）、7（5H）、6（6H）和1（7H）中分别检测到365、271、265、323、194和369个转录本。在小麦遗传背景中检测到1787个大麦转录本，并通过检测到的附加系（物理）定位到大麦染色体上。我们验证并扩展了我们的方法，将大麦基因物理映射到6号染色体（6H）的长臂和短臂。我们的物理图谱数据显示，大麦物理图谱与小麦和/或水稻同染色体的同源序列具有高度的同步性，这表明我们的大麦物理图谱是稳健的。结果表明，在小麦-大麦染色体附加系中检测大麦转录本是进行大规模基因物理定位的有效方法。
Wheat-barley chromosome addition lines are useful genetic resources for a variety of studies. In this study, transcript accumulation patterns in Betzes barley, Chinese Spring wheat, and Chinese Spring-Betzes chromosome addition lines were examined with the Barley1 Affymetrix GeneChip probe arrays Of the 4014 transcripts detected in Betzes but not in Chinese Spring, 365, 271, 265, 323, 194, and 369 were detected in wheat-barley disomic chromosome addition lines 2(2H), 3(3H), 4(4H), 7(5H), 6(6H), and 1(7H), respectively Thus, 1787 barley transcripts were detected in a wheat genetic background and, by virtue of the addition line in which the), were detected, were physical), mapped to barley chromosomes. We validated and extended our approach to physically map barley genes to the long and short arms of chromosome 6(6H). Our physical map data exhibited a high level of synteny with homologous sequences oil the wheat and/or rice syntenous chromosomes, indicating that our barley physical maps are robust. Our results show that barley transcript detection in wheat-barley chromosome addition lines is all efficient approach for large-scale physical mapping of genes.