High-resolution multistage MS, MS2, and MS3 matrix-assisted laser desorption/ionization FT-ICR mass spectra of peptides from a single laser shot.
High-resolution multistage MS, MS2, and MS3 matrix-assisted laser desorption/ionization FT-ICR mass spectra of peptides from a single laser shot.
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来自单次激光照射的肽的高分辨率多级 MS、MS2 和 MS3 基质辅助激光解吸/电离 FT-ICR 质谱。
DOI：
10.1021/ac960312d
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发表时间：
1996
影响因子：
7.4
通讯作者：
Marshall,AG
中科院分区：
文献类型：
--
作者：
Solouki,T;Pasa-Tolić,L;Jackson,GS;Guan,S;Marshall,AG
通过组合和重复使用持续非共振辐照(SORI)进行离子解离，存储波形反傅立叶变换(SWIFT)波形用于离子分离，以及离子轴化和重新测量技术，我们首次获得了从单一激光激发产生的多肽离子(马细胞色素的酶消化产物)的MS、MS2和MS3FT-ICR质谱图。从同一批离子中检测到的气相离子的连续碎裂增加了结构质谱学中痕量生物样品分析的灵敏度，并且通过解析碳-13同位素分布来促进碎片鉴定。通过对粗提纯的马细胞色素和牛细胞色素胰酶消化液样品的亚毫摩尔量的分析，说明了该方法的可行性。高分辨率的初级离子质谱图，以及碰撞诱导解离(CID)和傅立叶变换红外光谱(FT-ICR)的MSn功能，有助于确定碎片离子的初级氨基酸序列，而不是仅通过酶消化获得的序列，而不需要事先进行层析分离和纯化。
By combined and repeated use of sustained off-resonance irradiation (SORI) for ion dissociation, stored waveform inverse Fourier transform (SWIFT) waveforms for ion isolation, and ion axialization and remeasurement techniques, we obtain for the first time MS, MS2, and MS3FT-ICR mass spectra from peptide ions (enzymatic digest products of horse cytochromec) produced from asinglelaser shot. The successive fragmentation of gas-phase ions detected from thesameinitial batch of ions increases the sensitivity of analysis of trace amounts of biological samples in structural mass spectrometry, and fragment identification is facilitated by resolution of carbon-13 isotopic distributions. The method is illustrated by analyses of subfemtomole amounts of crudely purified samples of tryptic digest solutions of horse cytochromecand bovine cytochromec. The high-resolution primary ion mass spectrum, along with the collision-induced dissociation (CID) and MSncapabilities of FT-ICR, help to determine the primary amino acid sequence of the fragment ions beyond what is obtained from enzymatic digestion alone, without prior chromatographic separation and purification.