Expression of Sox11 in adult neurogenic niches suggests a stage-specific role in adult neurogenesis
Expression of Sox11 in adult neurogenic niches suggests a stage-specific role in adult neurogenesis
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DOI：
10.1111/j.1460-9568.2009.06768.x
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发表时间：
2009-06-01
影响因子：
3.4
通讯作者：
Lie, D. Chichung
中科院分区：
文献类型：
--
作者：
Haslinger, Anja;Schwarz, Tobias J.;Lie, D. Chichung
在哺乳动物的大脑中，侧脑室下区和齿状回亚颗粒区中的神经干和祖细胞在成年期产生新的神经元。新功能神经元的产生是一个复杂的过程，受外部信号的严格控制，并以阶段特异性基因表达程序和细胞生物学过程为特征。在成人神经发生中，调节这种特定阶段发育步骤的转录因子在很大程度上是未知的。在这里，我们报告了Sox11， C组Sox转录因子家族的成员，在成人大脑的神经原性区域显著表达。进一步分析表明，Sox11的表达严格局限于表达双皮质素的神经前体细胞和未成熟神经元，而Sox11不表达于表达sox2的非神经前体细胞和成年神经源性谱系的成熟神经元。最后，Sox11的过表达促进体外成人神经干细胞产生双皮质素阳性的未成熟神经元。这些数据表明，Sox11在未成熟神经元阶段参与了成人神经发生中特定基因表达程序的转录调控。
In the mammalian brain, neural stem and progenitor cells in the subventricular zone of the lateral ventricles and the subgranular zone of the dentate gyrus generate new neurons throughout adulthood. The generation of new functional neurons is a complex process that is tightly controlled by extrinsic signals and that is characterized by stage-specific gene expression programs and cell biological processes. The transcription factors regulating such stage-specific developmental steps in adult neurogenesis are largely unknown. Here we report that Sox11, a member of the group C Sox transcription factor family, is prominently expressed in the neurogenic areas of the adult brain. Further analysis revealed that Sox11 expression is strictly confined to doublecortin-expressing neuronally committed precursors and immature neurons but that Sox11 is not expressed in non-committed Sox2-expressing precursor cells and mature neurons of the adult neurogenic lineage. Finally, overexpression of Sox11 promotes the generation of doublecortin-positive immature neurons from adult neural stem cells in vitro. These data indicate that Sox11 is involved in the transcriptional regulation of specific gene expression programs in adult neurogenesis at the stage of the immature neuron.