Intra- and extracellular gradients of electrical potential and ion activities of the epithelial cells of the rabbit ileum in vivo recorded by microelectrodes.
Intra- and extracellular gradients of electrical potential and ion activities of the epithelial cells of the rabbit ileum in vivo recorded by microelectrodes.
复制标题
通过微电极记录体内兔回肠上皮细胞的细胞内和细胞外电位梯度和离子活性。
DOI：
10.1098/rstb.1975.0052
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发表时间：
1975
期刊：
影响因子：
--
通讯作者：
C. Monge
中科院分区：
文献类型：
--
作者：
T. Zeuthen;C. Monge
兔回肠上皮细胞长约40µm，直径约5µm。它们紧密排列成柱状，其粘膜末端面向肠腔，浆膜末端毗邻基膜，面向下面的毛细血管、淋巴管和结缔组织。肠道内壁覆盖着一层粘多糖，即黏液层。当Krebs溶液或类似的溶液被放置在管腔中时，众所周知（例如Edmonds 1970年的评论），溶液被输送到底层组织中。在运输过程中，肠道内的钠活性保持不变，氯活性降低，碳酸氢盐活性增加。然而，在这种运输过程中，每个细胞的胞内和胞外环境的关系却知之甚少。近年来，用于研究细胞在这种运输过程中状态的方法大多是需要在体外制备组织的方法，例如使用火焰光度法和usingchambers。就温血动物的肠道而言，选择这样的实验条件是不幸的，原因如下：(a)运输不再进入血液或淋巴引流系统，(b)上皮细胞必须从其管腔末端充氧，而在体内通常处于非常低的氧浓度（Crompton, Silver & Shrimpton 1965）和(c)在某些实验中，组织的生理参数在实验过程中发生变化（例如Powell， Binder & Curran 1973）。然而，微电极的使用允许在体内的方法，因为在测量期间组织可以留在其自然环境中。此外，在过去几年中，液体离子交换和离子敏感玻璃的发展导致了对各种离子选择性微电极的构建（Hinke 1959; Thomas 1970; Walker 1971），因此可以确定细胞内和细胞外电位和离子活性方面的细胞状态。因此，在离体牛蛙小肠上皮细胞中记录到了细胞内钠和钾的含量（Lee & Armstrong 1972）。本研究的目的首先是建立一种利用微电极研究兔回肠上皮细胞层的体内模型，其次是测定不同盐溶液填充小肠管腔时这些细胞的细胞内和细胞外电位和离子活性。
The epithelial cells of the rabbit ileum are about 40 µm long and have a diameter of roughly 5 µm. They are closely packed in a columnar fashion with their mucosal ends facing the lumen of the intestine and their serosal ends abutting the basement membrane and facing the underlying capillaries, lymphatics and connective tissue. The inner wall of the intestine is coated with a layer of mucopolysacharides, the mucus layer. When Krebs solution or similar solutions are placed in the lumen it is well known (e.g. the review by Edmonds 1970) that the solution is transported into the underlying tissues. During this transport the sodium activity in the gut lumen remains constant, the chloride activity decreases and the bicarbonate activity increases. However, the relationship of the intra- to the extracellular environment of each individual cell during this transport is only little understood. In recent years the methods applied in the study of the state of the cells during this transport have mostly been of a type that requires preparation of the tissue in vitro , e.g. usage of flamephotometry and Ussing-chambers. In case of the intestine of warm-blooded animals such a choice of experimental conditions is unfortunate for several reasons: ( a ) The transport is no longer into the blood or lymphatic drainage, ( b ) the epithelial cells have to be oxygenated from their luminal ends which in vivo are normally at a very low oxygen concentration (Crompton, Silver & Shrimpton 1965) and ( c ) in some experiments the physiological parameters of the tissue change during the experiment (e.g. Powell, Binder & Curran 1973). However, the use of microelectrodes allows an in vivo approach since the tissue can be left in its natural environment during the measurements. Furthermore liquid ion exchanges and ion-sensitive glasses developed over the past few years have led to the construction of microelectrodes selective to various ions (Hinke 1959; Thomas 1970; Walker 1971), so that the state of cells in terms of intra- and extracellular electrical potential and ion activities can be determined. Thus intracellular sodium and potassium has been recorded in the epithelial cells of the bullfrog small intestine in vitro (Lee & Armstrong 1972). The purpose of this work was first to construct an in vivo model in which the epithelial cell layer of the rabbit ileum could be studied by microelectrodes and secondly to determine the intra and extracellular electrical potential and ion activities in these cells when the lumen of the intestine was filled with various salt solutions.