Creation of saccular aneurysms in the rabbit: A model suitable for testing endovascular devices
Creation of saccular aneurysms in the rabbit: A model suitable for testing endovascular devices
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DOI：
10.2214/ajr.174.2.1740349
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发表时间：
2000-02-01
影响因子：
5
通讯作者：
Kallmes, DF
中科院分区：
文献类型：
--
作者：
Altes, TA;Cloft, HJ;Kallmes, DF
目标。本研究建立了一种颅内动脉瘤动物模型，适用于评估新出现的动脉瘤治疗血管内装置。我们描述了这种血管内技术用于兔囊状动脉瘤形成的短期、中期和长期特性。材料和方法。9只新西兰大白兔手术暴露右侧颈总动脉。使用血管内技术，我们用一个柔软的球囊闭塞右颈总动脉的起源。弹力酶经腔内孵育于球囊上方的颈总动脉近端。远端结扎颈总动脉。动物在动脉瘤形成后2周（n = 3）、10周（n = 3）和24周（n = 3）进行血管造影研究并处死。进行组织学检查。9只兔子中有8只形成了囊状动脉瘤。动脉瘤从母血管——头臂动脉的约90度曲线的顶点凸出。动脉瘤平均直径为4.5 mm (SD, 1.2 mm)，平均高度为7.5 mm （SD, 1.6 mm）。所有样本均显示弹性层变薄，无炎症迹象。在8例动脉瘤中，有4例在瘤顶存在无组织血栓。在高剪切应力区可靠地形成内皮完整、弹性层缺失的新西兰大白兔动脉动脉瘤。其中3个动脉瘤至少存活了6个月。我们发现了一个简单的程序，可以很容易地应用于测试新的血管内装置，以可靠地在兔子身上产生动脉瘤。
OBJECTIVE. This study developed an animal model of intracranial aneurysms suitable for evaluating emerging endovascular devices for aneurysmal therapy. We characterized the short-, medium-, and long-term attributes of this endovascular technique for saccular aneurysmal creation in the rabbit.MATERIALS AND METHODS. The right common carotid artery was surgically exposed in nine New Zealand white rabbits. Using endovascular techniques, we occluded the origin of the right common carotid artery with a pliable balloon. Elastase was incubated endoluminally in the proximal common carotid artery above the balloon. The common carotid artery was ligated distally. Animals were studied angiographically and sacrificed at 2 weeks (n = 3), 10 weeks (n = 3), and 24 weeks (n = 3) after aneurysm creation. Histology was obtained.RESULTS. Saccular aneurysms formed in eight of the nine rabbits. The aneurysm projected from the apex of an approximately 90 degrees curve of the parent vessel, the brachiocephalic artery. Mean aneurysm diameter was 4.5 mm (SD, 1.2 mm), and mean height was 7.5 mm (SD, 1.6 mm). All samples showed thinned elastic lamina and no evidence of inflammation. in four of eight aneurysms, unorganized thrombus was present in the dome of the aneurysm.CONCLUSION. Arterial aneurysms with intact endothelium and deficient elastic lamina were reliably created in an area of high shear stress in New Zealand white rabbits. Three of these aneurysms remained patent for at least 6 months. We found a simple procedure that can be readily applied to the testing of new endovascular devices for a reliable creation of aneurysms in rabbits.