Immunological properties of gold nanoparticles.
Immunological properties of gold nanoparticles.
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DOI：
10.1039/c6sc03631g
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发表时间：
2017-03-01
期刊：
影响因子：
8.4
通讯作者：
Khlebtsov NG
中科院分区：
文献类型：
--
作者：
Dykman LA;Khlebtsov NG
本文综述了金纳米颗粒作为抗原载体和佐剂在体内免疫制备抗体和评估其发展有效疫苗的潜力的应用。在过去的十年中，金纳米粒子引起了强烈的兴趣，从纳米生物技术界由于在强大的和易于制作的合成技术，在表面功能化，并在有前途的生物医学应用取得了重大进展。这些包括生物成像、基因诊断、分析传感、肿瘤的光热治疗以及各种生物分子和化学货物的靶向递送。对于最后一种应用，金纳米颗粒应该被适当地制造，以通过有效的内吞作用将货物递送到靶细胞中。在这篇综述中，我们讨论了最近的进展，了解金纳米粒子的选择性渗透到免疫细胞。金纳米粒子与免疫细胞受体的相互作用进行了讨论。不同于其他已发表的评论，我们提出了一个总结金纳米粒子的免疫学特性。本文还总结了金纳米粒子作为抗原载体和佐剂在体内免疫制备抗体中的应用。对于上述每个主题，讨论了基本原则，最近的进展和当前的挑战。因此，本文综述了金纳米粒子与免疫细胞相互作用的数据的详细分析。重点放在通过使用金纳米颗粒和金纳米颗粒的佐剂性质生产抗体的数据的系统化。具体来说，我们从目前各种金纳米颗粒与免疫细胞相互作用的数据开始讨论。下一部分描述了通过使用与特定配体缀合的金纳米颗粒来改善体内抗体产生的现有技术。最后，我们描述了什么是已知的裸金或功能化的纳米粒子的辅助性能。在结论部分，我们对报告的数据进行了简短总结，并提出了一些挑战和观点。
This review summarizes what is known about the application of gold nanoparticles as an antigen carrier and adjuvant in immunization for the preparation of antibodies in vivo and evaluating their potential for the development of effective vaccines. In the past decade, gold nanoparticles have attracted strong interest from the nanobiotechnological community owing to the significant progress made in robust and easy-to-make synthesis technologies, in surface functionalization, and in promising biomedical applications. These include bioimaging, gene diagnostics, analytical sensing, photothermal treatment of tumors, and targeted delivery of various biomolecular and chemical cargos. For the last-named application, gold nanoparticles should be properly fabricated to deliver the cargo into the targeted cells through effective endocytosis. In this review, we discuss recent progress in understanding the selective penetration of gold nanoparticles into immune cells. The interaction of gold nanoparticles with immune cell receptors is discussed. As distinct from other published reviews, we present a summary of the immunological properties of gold nanoparticles. This review also summarizes what is known about the application of gold nanoparticles as an antigen carrier and adjuvant in immunization for the preparation of antibodies in vivo. For each of the above topics, the basic principles, recent advances, and current challenges are discussed. Thus, this review presents a detailed analysis of data on interaction of gold nanoparticles with immune cells. Emphasis is placed on the systematization of data over production of antibodies by using gold nanoparticles and adjuvant properties of gold nanoparticles. Specifically, we start our discussion with current data on interaction of various gold nanoparticles with immune cells. The next section describes existing technologies to improve production of antibodies in vivo by using gold nanoparticles conjugated with specific ligands. Finally, we describe what is known about adjuvant properties of bare gold or functionalized nanoparticles. In the Conclusion section, we present a short summary of reported data and some challenges and perspectives.