Integrin distribution in malignant melanoma: association of the beta 3 subunit with tumor progression.
Integrin distribution in malignant melanoma: association of the beta 3 subunit with tumor progression.
复制标题
DOI：
--
复制
发表时间：
1990-10
期刊：
影响因子：
11.2
通讯作者：
S. Albelda;S. Mette;D. Elder;R. Stewart;L. Damjanovich;M. Herlyn;C. Buck
中科院分区：
文献类型：
--
作者：
S. Albelda;S. Mette;D. Elder;R. Stewart;L. Damjanovich;M. Herlyn;C. Buck
由于肿瘤进展依赖于恶性细胞与细胞外基质相互作用的能力，因此细胞表面介导细胞-基质相互作用的分子可能是肿瘤侵袭和转移的重要调节因子。本研究的目的是研究一类细胞粘附受体整合素在人类黑素细胞良恶性病变中的分布。利用一组针对特定整合素亚单位的单克隆抗体，确定了整合素粘附受体在正常和恶性黑色素细胞培养细胞和良性到恶性黑色素细胞病变组织切片上的分布。培养的细胞表达多种整合素，包括所有先前表征的β 1亚家族成员以及α v/ β 3玻璃体连接蛋白受体。整合素在良性和恶性病变细胞中的表达相似。相比之下，与放射生长期黑色素瘤细胞和痣内细胞相比，转移性和垂直生长期黑色素瘤组织内细胞的整合素表达存在一致的差异。最值得注意的是，β 3亚基的表达仅限于垂直生长期和转移性黑色素瘤的细胞。这种整合素的存在在肿瘤侵袭性的发展中可能是重要的，并且可以作为黑色素瘤细胞进入恶性过程更具侵袭性阶段的标记物。
Since tumor progression is dependent on the ability of malignant cells to interact with the extracellular matrix, molecules on the cell surface which mediate cell-substratum interactions are likely to be important regulators of tumor invasion and metastasis. The purpose of this study was to examine the distribution of one such group of cell adhesion receptors, the integrins, in benign and malignant lesions of human melanocytes. The distribution of integrin adhesion receptors was defined on cells in culture derived from normal and malignant melanocytes and in tissue sections from benign to increasingly malignant melanocytic lesions using a panel of monoclonal antibodies against specific integrin subunits. Cells in culture expressed a large variety of integrins, including all of the previously characterized members of the beta 1 subfamily plus the alpha v/beta 3 vitronectin receptor. The expression of integrins was similar in cells cultured from either benign or malignant lesions. In contrast, consistent differences were noted in integrin expression by cells within tissues containing metastatic and vertical growth phase melanomas when compared to radial growth phase melanoma cells and cells within nevi. Most notably, the expression of the beta 3 subunit was restricted exclusively to cells within vertical growth phase and metastatic melanomas. The presence of this integrin may be important in the development of tumor invasiveness and could be useful as a marker of melanoma cells entering the more aggressive phase of the malignant process.