On-demand serum-degradable amylopectin-based in situ gellable hydrogel
On-demand serum-degradable amylopectin-based in situ gellable hydrogel
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DOI：
10.1039/c1jm14460j
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发表时间：
2012-01
影响因子：
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通讯作者：
S. Sakai;Yang Liu;Tomohiro Matsuyama;K. Kawakami;M. Taya
中科院分区：
文献类型：
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作者：
S. Sakai;Yang Liu;Tomohiro Matsuyama;K. Kawakami;M. Taya
本文描述了可通过暴露于血清而快速降解（甚至按需降解）的水凝胶，以及在温和条件下可用于细胞的原位胶凝。此外，这些水凝胶在组织工程和再生医学的潜在应用也被描述。通过辣根过氧化物酶催化的反应，从支链淀粉衍生物的水溶液中产生的酚羟基部分（AP-Ph）的水凝胶。在血清存在下的降解速率增强与胎牛血清（FBS）的含量增加，并减少在AP-Ph. A 1 mm厚的水凝胶封闭小鼠胚胎fibroblastSTO细胞制备条件下，在约30秒的凝胶化在FBS中孵育3小时内消失的Ph部分的含量。从水凝胶释放的细胞附着在培养皿上，并显示出与未被封闭在水凝胶中的那些接种的细胞相似的形态。此外，在无血清培养基中在基于AP-Ph的水凝胶上培养的汇合的人上皮细胞可以通过浸泡在FBS中在10分钟内收获，而不分离成单个细胞。这些结果表明，原位凝胶化的血清可降解的AP-Ph水凝胶可能是一个有前途的新工具，用于组织工程。
This paper describes the hydrogels which are quickly degradable, even on-demand, by exposure to serum, as well as in situ gellable under mild conditions for cells. In addition, potential applications of these hydrogels to tissue engineering and regenerative medicine are also described. The hydrogels were produced from aqueous solution of amylopectin derivatives bearing phenolic hydroxyl moieties (AP-Ph) via horseradish peroxidase-catalysed reaction. The degradation rate in the presence of serum was enhanced with increasing the content of fetal bovine serum (FBS) and decreasing the content of Ph moieties in AP-Ph. A 1 mm thick hydrogel enclosing mouse embryonic fibroblastSTO cells prepared under conditions giving gelation in about 30 s disappeared within 3 h of incubation in FBS. The cells released from the hydrogel attached onto a culture dish and showed similar morphology to those seeded without being enclosed in the hydrogels. Furthermore, confluent human epithelial cells cultured on the AP-Ph-based hydrogel in serum-free medium can be harvested within 10 min by soaking in FBS while without separating into individual cells. These results indicate that the in situ gellable serum-degradable AP-Ph hydrogels may be a promising new tool for tissue engineering.