Formation of colony patterns by a bacterial cell population
Formation of colony patterns by a bacterial cell population
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DOI：
10.1016/s0378-4371(99)00328-3
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发表时间：
1999-12-01
期刊：
影响因子：
3.3
通讯作者：
Mimura, M
中科院分区：
文献类型：
--
作者：
Matsushita, M;Wakita, J;Mimura, M
已知细菌物种枯草芽孢杆菌（Bacillus subtilis）表现出各种菌落模式，如扩散限制聚集（DLA）样、紧凑Eden样、密集分支形态（DBM）样、同心环状和盘状，这取决于基质柔软度和营养物浓度。我们已经建立了菌落模式的形态图，并在宏观和微观上研究和表征它们如何生长。例如，我们发现似乎有两种细菌细胞;活性细胞和非活性细胞，前者驱动集落界面向外，活性细胞与非活性细胞在DBM样集落的生长分支的尖端处特别区分，作为特征性指甲结构，我们还发现，同心环状集落的形成是由活性细胞组成的生长界面交替重复前进和静止的结果。根据我们的观察，我们已经建立了一个现象学的，但统一的模型，产生特征的殖民地模式。这是一个反应扩散型的细菌细胞的种群密度和营养浓度的模型。基本的假设是存在两种类型的细菌细胞：活跃的细胞，活跃地移动，生长和进行细胞分裂，以及不活动的细胞。我们的模型被发现能够在全球范围内再现实验获得的形态图中看到的所有殖民地模式，并且在现象学上是相当令人满意的，（C）1999 Elsevier Science B. V.保留所有权利。
Bacterial species Bacillus subtilis is known to exhibit various colony patterns, such as diffusion-limited aggregation (DLA)-like, compact Eden-like, dense branching morphology (DBM)-like, concentric ring-like and disk-like, depending on the substrate softness and nutrient concentration. We have established the morphological diagram of colony patterns, and examined and characterized both macroscopically and microscopically how they grow. For instance, we have found that there seem to be two kinds of bacterial cells; active and inactive cells, the former of which drive colony interfaces outward, The active cells are particularly distinguished from the inactive ones at the tips of growing branches of a DBM-like colony as the characteristic fingernail structure, We have also found that the concentric ring-like colony is formed as a consequence of alternate repetition of advancing and resting of the growing interface which consists of active cells. Based on our observations, we have constructed a phenomenological but unified model which produces characteristic colony patterns. It is a reaction-diffusion type model for the population density of bacterial cells and the concentration of nutrient. The essential assumption is that there exist: two types of bacterial cells; active cells that move actively, grow and perform cell division, and inactive ones that do nothing at all. Our model is found to be able to reproduce globally all the colony patterns seen in the experimentally obtained morphological diagram, and is phenomenologically quite satisfactory, (C) 1999 Elsevier Science B.V. All rights reserved.