SELF-RESTRICTED PRIMARY HUMAN HISTOCOMPATIBILITY LEUKOCYTE ANTIGEN (HLA)-SPECIFIC CYTOTOXIC LYMPHOCYTES-T
SELF-RESTRICTED PRIMARY HUMAN HISTOCOMPATIBILITY LEUKOCYTE ANTIGEN (HLA)-SPECIFIC CYTOTOXIC LYMPHOCYTES-T
复制标题
DOI：
10.1093/intimm/5.1.103
复制
发表时间：
1993-01-01
影响因子：
4.4
通讯作者：
IVANYI, P
中科院分区：
文献类型：
--
作者：
BREURVRIESENDORP, BS;IVANYI, P
自身MHC限制性T细胞对加工抗原的肽片段应答的特异性已经得到很好的表征。然而，同种异体反应性T淋巴细胞识别MHC同种异体抗原的方法仍然知之甚少。我们现在提供的证据表明，原代混合淋巴细胞培养物中诱导的同种反应性细胞毒性T淋巴细胞（CTL）对I类人类白细胞抗原（HLA）同种抗原的识别在很大程度上受到I类HLA的限制。这通过使用携带刺激性I类HLA同种异体抗原的第三方靶细胞的“分孔”有限稀释测定显示，所述刺激性I类HLA同种异体抗原（i）存在或（ii）不存在与应答者共有的I类HLA抗原。发现同种异体反应性CTL识别第一类靶细胞上的刺激因子I类HLA同种异体抗原，但不识别第二类靶细胞上的刺激因子I类HLA同种异体抗原。结果表明，与传统观点相反，在原代混合淋巴细胞培养中产生的针对I类HLA同种异体抗原的同种异体反应性CTL中有很大比例是自身I类HLA限制性的。
The specificity of self MHC-restricted T cells responding to peptide fragments of processed antigen has been well characterized. However, the means by which alloreactive T lymphocytes recognize MHC alloantigen remain poorly understood. We now provide evidence that the recognition of class I human leukocyte antigen (HLA) alloantigen by alloreactive cytotoxic T lymphocytes (CTL) induced in primary mixed lymphocyte cultures is class I HLA-restricted to a great extent. This is shown by the 'split-well' limiting dilution assay using third party target cells bearing a stimulating class I HLA alloantigen either in (i) the presence or (ii) the absence of class I HLA antigen(s) which is shared with the responder. Alloreactive CTL were found which recognize stimulator class I HLA alloantigen on the first but not on the second type of target cells. The results show that, in contrast to the traditional view, a large proportion of the alloreactive CTL against class I HLA alloantigen generated in primary mixed lymphocyte cultures is self class I HLA-restricted.