Rational Design of a Novel Propeptide for Improving Active Production of Streptomyces griseus Trypsin in Pichia pastoris
Rational Design of a Novel Propeptide for Improving Active Production of Streptomyces griseus Trypsin in Pichia pastoris
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合理设计一种提高毕赤酵母灰色链霉菌胰蛋白酶活性的新型前肽
DOI：
10.1128/aem.00376-13
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发表时间：
2013-06-01
影响因子：
4.4
通讯作者：
Du, Guocheng
中科院分区：
文献类型：
--
作者：
Ling, Zhenmin;Liu, Yi;Du, Guocheng
通过计算机模拟和体外实验，证明了氨基酸脯氨酸对灰色链霉菌胰蛋白酶原的影响，并发现H-57和D-102之间的氢键对胰蛋白酶活性起着至关重要的作用。通过引入人工前肽IVEF，胰蛋白酶的效价提高了6.71倍。
Applying in silico simulations and in vitro experiments, the amino acid proline was proved to have a profound influence on Streptomyces griseus trypsinogen, and the hydrogen bond between H-57 and D-102 was found to be crucial for trypsin activity. By introducing an artificial propeptide, IVEF, the titer of trypsin was increased 6.71-fold.