Solving the "world-to-chip" interface problem with a microfluidic matrix
Solving the "world-to-chip" interface problem with a microfluidic matrix
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DOI：
10.1021/ac0346407
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发表时间：
2003-09-15
影响因子：
7.4
通讯作者：
Quake, SR
中科院分区：
文献类型：
--
作者：
Liu, J;Hansen, C;Quake, SR
我们报告了宏观/微流体界面问题的有效解决方案，并展示了微流体如何在减少移液操作的复杂性方面实现令人印象深刻的规模经济。使用N × N微流体基质，N = 20，我们只需要2N + 1 = 41个移液步骤，就可以进行N-2 = 400个不同的PCR反应，而传统的流体处理需要3N(2) = 1200个步骤。矩阵的每个顶点都有一个3-nL的反应器，单个2-muL的聚合酶分摊到所有400个独立的反应中，从而大大减少了样品开销并最大限度地减少了试剂消耗。除了PCR之外，基质芯片还提供了一种通用的方法，以高度自动化的方式使用珍贵的试剂进行化学和生物实验。
We report an effective solution to the macroscopic/ microfluidic interface issue and demonstrate how microfluidics can achieve impressive economies of scale in reducing the complexity of pipetting operations. Using an N x N microfluidic matrix with N = 20, we performed N-2 = 400 distinct PCR reactions with only 2N + 1 = 41 pipetting steps, compared with the 3N(2) = 1200 steps required with conventional fluid handling. Each vertex of the matrix has a 3-nL reactor, and a single 2-muL aliquot of polymerase is amortized over all 400 independent reactions, thus dramatically reducing sample overhead and minimizing reagent consumption. Beyond PCR, the matrix chip provides a general method to perform chemical and biological experiments with precious reagents in a highly automated fashion.