Laser spray: electric field-assisted matrix-assisted laser desorption/ionization.
Laser spray: electric field-assisted matrix-assisted laser desorption/ionization.
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DOI：
10.1002/jms.621
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发表时间：
2004-04
期刊：
影响因子：
--
通讯作者：
K. Hiraoka
中科院分区：
文献类型：
--
作者：
K. Hiraoka
一种新的液相色谱/质谱接口--激光喷雾已经开发出来。当从不锈钢毛细管的尖端流出的水溶液被10.6微米红外激光从毛细管的相对侧照射时，发生爆炸性蒸发和雾形成。通过离子采样孔对羽流进行采样，可以检测到微弱的离子信号。当高电压（3-4 kV）施加到不锈钢毛细管时，出现强离子信号。的离子丰度被发现是数量级大于那些通过传统的电喷雾电离的情况下，水溶液。本方法被认为是一种电场辅助形式的基质辅助激光解吸/电离，其中液相色谱溶剂（水等）充当液体基质。激光喷雾离子化技术是一种适用于生物质谱分析的通用方法，因为该方法与天然溶剂水相容。
A new liquid chromatography/mass spectrometry interface, the laser spray, has been developed. Explosive vaporization and mist formation occur when an aqueous solution effusing out from the tip of the stainless-steel capillary is irradiated from the opposite side of the capillary by a 10.6 microm infrared laser. Weak ion signals could be detected when the plume was sampled through the ion sampling orifice. When a high voltage (3-4 kV) was applied to the stainless-steel capillary, strong ion signals appeared. The ion abundances were found to be orders of magnitude greater than those obtained by conventional electrospray ionization in the case of aqueous solutions. The present method is regarded as an electric-field assisted form of matrix-assisted laser desorption/ionization in which the liquid chromatographic solvent (water, etc.) acts as a liquid matrix. Laser spray ionization is expected to become a versatile method for biological mass spectrometry because this method is compatible with the natural solvent, water.