Differential soluble protein expression between Trichomonas vaginalis isolates exhibiting low and high virulence phenotypes
Differential soluble protein expression between Trichomonas vaginalis isolates exhibiting low and high virulence phenotypes
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DOI：
10.1016/j.jprot.2008.01.010
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发表时间：
2008-04-30
影响因子：
3.3
通讯作者：
De Jesus, Jose Batista
中科院分区：
文献类型：
--
作者：
Cuervo, Patricia;Cupolillo, Elisa;De Jesus, Jose Batista
采用二维凝胶电泳和质谱法对长期生长和新鲜临床分离的阴道毛滴虫分别表现出低毒力和高毒力表型的蛋白质组学图进行了比较分析。在29个差异表达的蛋白点中，有19个在分离物中过度表达，表现出高毒力表型：与细胞骨架动力学相关的蛋白质，如冠状蛋白和肌动蛋白的几种亚型，以及参与信号转导、蛋白质转换、蛋白质水解、能量和多胺代谢的蛋白质。一些苹果酸脱氢酶、果糖-1,6-二磷酸醛缩酶和鸟氨酸环脱酰胺酶的异构体被高毒力的分离株完全表达。在与VEC的相互作用试验中，表现出高毒力表型的寄生虫迅速粘附并转变为变形虫形式。而在低毒力表型的寄生虫中，没有观察到低粘附和形态转化。我们的研究结果表明，高毒力和低毒力寄生虫的特定蛋白表达可能与每种分离物进行形态转化和与宿主细胞相互作用的能力有关。这些数据是了解参与这种原生动物发病机制的蛋白质复杂相互作用网络的重要一步。(C) 2008 Elsevier B.V.版权所有
A comparative analysis of proteomic maps of long-term grown and fresh clinical Trichomonas vaginalis isolates exhibiting low and high virulence phenotypes, respectively, was performed using two-dimensional gel electrophoresis and mass spectrometry. Of 29 protein spots differentially expressed between the isolates, 19 were over-expressed in the isolate exhibiting high virulence phenotype: proteins associated with cytoskeletal dynamics, such as coronin and several isoforms of actin, as well as proteins involved in signal transduction, protein turnover, proteolysis, and energetic and polyamine metabolisms were identified. Some malate dehydrogenase, fructose-1,6-bisphosphate aldolase and ornithine cyclodeamidase isoforms were exclusively expressed by the highly virulent isolate. During interaction assays with VEC, parasites exhibiting high virulence phenotype rapidly adhered and switched to amoeboid forms. In contrast, low adhesion and no morphological transformation were observed in parasites displaying low virulence phenotype. our findings demonstrate that expression of specific proteins by high and low virulence parasites could be associated with the ability of each isolate to undergo morphological transformation and interact with host cells. Such data represent an important step towards understanding of the complex interaction network of proteins that participate in the mechanism of pathogenesis of this protozoan. (C) 2008 Elsevier B.V. All rights reserved.