Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly
Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly
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DOI：
10.3390/mi10010068
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发表时间：
2019-01-01
期刊：
影响因子：
3.4
通讯作者：
Douglas, Timothy E. L.
中科院分区：
文献类型：
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作者：
Norris, Karl;Mishukova, Oksana I.;Douglas, Timothy E. L.
海洋来源的多糖作为生物材料成分正受到越来越多的关注。来自深海热液喷口的细菌可以产生硫酸化的胞外多糖(EPS)，这可能会影响细胞的行为。这种多糖作为生物材料表面的有机、胶原纤维涂层的组成部分的用途仍未被探索。本研究通过快速成型技术在钛合金(Ti6Al4V)支架上制备了富含HE800和GY785 EPS衍生物的胶原原纤维涂层，并对涂层进行了理化和细胞生物学表征。涂层是通过在酸性胶原分子溶液中加入多糖，然后中和诱导胶原纤维自组装而形成的。纤维的形成导致胶原水凝胶的形成。水凝胶直接在Ti6Al4V表面形成，纤维吸附在表面。扫描电子显微镜(SEM)分析显示，胶原原纤维涂层中的多糖与原纤维之间存在相互作用。细胞生物学特性显示细胞在裸露的Ti6Al4V表面有良好的黏附和生长，仅有胶原纤维涂层和经HE800和GY785 EPS衍生物增强的胶原纤维涂层。因此，使用这两种EPS衍生物作为涂料组分是可行的。进一步的工作应该集中在细胞分化上。
Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored. In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization. Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils. Fibril formation resulted in collagen hydrogel formation. Hydrogels formed directly on Ti6Al4V surfaces, and fibrils adsorbed onto the surface. Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils. Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives. Hence, the use of both EPS derivatives as coating components is feasible. Further work should focus on cell differentiation.