Quality Assessment of Urinary Stone Analysis: Results of a Multicenter Study of Laboratories in Europe
Quality Assessment of Urinary Stone Analysis: Results of a Multicenter Study of Laboratories in Europe
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DOI：
10.1371/journal.pone.0156606
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发表时间：
2016-06-01
期刊：
影响因子：
3.7
通讯作者：
Trinchieri, Alberto
中科院分区：
文献类型：
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作者：
Siener, Roswitha;Buchholz, Noor;Trinchieri, Alberto
结石清除后，准确分析泌尿系结石成分是结石形成患者治疗和预防复发的最重要的实验室诊断程序。最常见的宝石常规分析技术是红外光谱、X射线衍射和化学分析。本研究的目的是评估欧洲实验室尿结石分析的质量。2010年至2014年，来自8个欧洲国家的9个实验室参与了德国波恩生物分析参考研究所的6项尿结石分析质量控制调查。每名参与者接受相同的盲态试验样本进行结石分析。共分析了24个样品，包括纯物质和两种或三种成分的混合物。在本研究中，实验室质量的评价是基于达到最高总分的75%，即99分。所用的结石分析方法为红外光谱法（n = 7）、化学分析法（n = 1）和X射线衍射法（n = 1）。在目前的研究中，只有56%的实验室，四个使用红外光谱，一个使用X射线衍射，满足质量要求。根据目前的标准，化学分析被认为是不足以进行石材分析，而红外光谱或X射线衍射是强制性的。然而，红外光谱的不良结果突出了设备、参考光谱和工作人员资格对于准确分析石材成分的重要性。定期的质量控制在进行常规结石分析中至关重要。
After stone removal, accurate analysis of urinary stone composition is the most crucial laboratory diagnostic procedure for the treatment and recurrence prevention in the stone-forming patient. The most common techniques for routine analysis of stones are infrared spectroscopy, X-ray diffraction and chemical analysis. The aim of the present study was to assess the quality of urinary stone analysis of laboratories in Europe. Nine laboratories from eight European countries participated in six quality control surveys for urinary calculi analyses of the Reference Institute for Bioanalytics, Bonn, Germany, between 2010 and 2014. Each participant received the same blinded test samples for stone analysis. A total of 24 samples, comprising pure substances and mixtures of two or three components, were analysed. The evaluation of the quality of the laboratory in the present study was based on the attainment of 75% of the maximum total points, i.e. 99 points. The methods of stone analysis used were infrared spectroscopy (n = 7), chemical analysis (n = 1) and X-ray diffraction (n = 1). In the present study only 56% of the laboratories, four using infrared spectroscopy and one using X-ray diffraction, fulfilled the quality requirements. According to the current standard, chemical analysis is considered to be insufficient for stone analysis, whereas infrared spectroscopy or X-ray diffraction is mandatory. However, the poor results of infrared spectroscopy highlight the importance of equipment, reference spectra and qualification of the staff for an accurate analysis of stone composition. Regular quality control is essential in carrying out routine stone analysis.