Micro-patterned surfaces based on plasma modification of PEO-like coating for biological applications
Micro-patterned surfaces based on plasma modification of PEO-like coating for biological applications
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DOI：
10.1016/j.snb.2006.08.040
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发表时间：
2007-04-10
影响因子：
8.4
通讯作者：
Rossi, Francois
中科院分区：
文献类型：
--
作者：
Bretagnol, Frederic;Kylian, Ondrej;Rossi, Francois
在这项研究中，描述了用于生产微结构表面的等离子体和光刻工艺的组合。第一步，通过二甘醇二甲醚的脉冲等离子体聚合获得对细胞和蛋白质不粘附的层。第二步将借助光刻技术生成图案和通过第二次等离子体处理激活第一层来制造生物粘附区域。通过氩-氢混合物的后等离子体放电来实现活化，这允许控制涂层的醚键浓度，而无需在表面引入新的反应性化学部分（例如羟基或羧基）。在处理过的表面上对模型蛋白（牛血清白蛋白）和 L929 小鼠成纤维细胞进行的实验表明，Ar/H-2 等离子体处理适合调整其生物粘附特性。此外，通过这种等离子体工艺组合获得的微结构表面可以实现蛋白质吸附和细胞附着的空间控制。 (c) 2006 Elsevier B.V. 保留所有权利。
In this study, a combination of plasma and photolithographic processes for production of micro-structured surfaces is described. In a first step, a layer which is non-adhesive towards cells and proteins is obtained by pulsed plasma polymerization of Diethylene Glycol Dimethyl Ether. The second step combines pattern generation with the help of photolithography and fabrication of bio-adhesive areas by activation of the first layer by a second plasma treatment. The activation is obtained with a post-plasma discharge of an argon-hydrogen mixture, which permits to control the concentration of ether bonds of the coatings without introducing new reactive chemical moieties (e.g. hydroxyl or carboxyl) at the surface. Experiments with a model protein (Bovine Serum Albumin) and L929 mouse fibroblast cells on treated surfaces show that Ar/H-2 plasma treatment is suitable for tuning their bio-adhesive properties. Moreover, micro-structured surfaces obtained by this combination of plasma processes allow the spatial control of protein adsorption and cell attachment. (c) 2006 Elsevier B.V. All rights reserved.