Ventilation strategy has a major influence on remote ischaemic preconditioning in mice.
Ventilation strategy has a major influence on remote ischaemic preconditioning in mice.
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DOI：
10.1111/jcmm.13164
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发表时间：
2017-10
影响因子：
5.3
通讯作者：
Yellon DM
中科院分区：
文献类型：
--
作者：
Davidson SM;He Z;Dyson A;Bromage DI;Yellon DM
ST段抬高型心肌梗死期间是否应该急性给氧是有争议的。尽管存在争议，但补充氧对缺血再灌注损伤或心脏保护的动物模型可能产生的影响很少被考虑。我们使用小鼠体内缺血再灌注模型来研究室内空气与100%氧气通气的影响。麻醉小鼠冠状动脉闭塞40分钟，再灌注2小时。用四氮唑染色法测量梗死面积，用埃文蓝法表示为危险面积的百分比。出乎意料的是，100%氧气通气小鼠的梗死面积明显小于室内空气通气小鼠（33±5% vs 46±3%;n = 6; P < 0.01）。我们测试了3 × 5分钟循环远程缺血预处理（RIPC）的标准方案，发现它不能保护100%氧气通气的小鼠。RIPC协议使用2.5分钟或10分钟。在给氧通气的小鼠中，闭塞同样无效。直接心脏缺血预处理获得了类似的完全不同的结果。相比之下，再灌注时给予缓激肽的药理保护即使在100%氧气通气的小鼠中也有效，将梗死面积从33±5%减少到21±3% （n = 4-6; P < 0.01）。在后肢进行了血流激光散斑对比成像和直接pO2测量，但这些测量与保护无关。综上所述，通气方案对小鼠梗死面积和缺血预处理方案有重要影响。
Whether oxygen should be administered acutely during ST‐segment elevation myocardial infarction is debated. Despite this controversy, the possible influence of supplementary oxygen on animal models of ischaemia–reperfusion injury or cardioprotection is rarely considered. We used an in vivo mouse model of ischaemia and reperfusion to investigate the effect of ventilation with room air versus 100% oxygen. The coronary artery of anaesthetized mice was occluded for 40 min. followed by 2‐hrs reperfusion. Infarct size was measured by tetrazolium staining and expressed as a percentage of area at risk, determined using Evan's blue. Unexpectedly, infarct size in mice ventilated with 100% oxygen was significantly smaller than in those ventilated with room air (33 ± 5% versus 46 ± 3%; n = 6; P < 0.01). We tested a standard protocol of 3 × 5 min. cycles of remote ischaemic preconditioning (RIPC) and found this was unable to protect mice ventilated with 100% oxygen. RIPC protocols using 2.5‐ or 10‐min. occlusion were similarly ineffective in mice ventilated with oxygen. Similar disparate results were obtained with direct cardiac ischaemic preconditioning. In contrast, pharmacological protection using bradykinin administered at reperfusion was effective even in mice ventilated with 100% oxygen, reducing infarct size from 33 ± 5% to 21 ± 3% (n = 4–6; P < 0.01). Laser speckle contrast imaging of blood flow and direct pO2 measurements were made in the hindlimb, but these measurements did not correlate with protection. In conclusion, ventilation protocol can have a major influence on infarct size and ischaemic preconditioning protocols in mice.
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影响因子：
9.5
作者：
Pickard JM;Bøtker HE;Crimi G;Davidson B;Davidson SM;Dutka D;Ferdinandy P;Ganske R;Garcia-Dorado D;Giricz Z;Gourine AV;Heusch G;Kharbanda R;Kleinbongard P;MacAllister R;McIntyre C;Meybohm P;Prunier F;Redington A;Robertson NJ;Suleiman MS;Vanezis A;Walsh S;Yellon DM;Hausenloy DJ
通讯作者：
Hausenloy DJ
影响因子：
9.9
作者：
Singhal, AB;Dijkhuizen, RM;Lo, EH
通讯作者：
Lo, EH
影响因子：
9.5
作者：
Lim, Shiang Y.;Yellon, Derek M.;Hausenloy, Derek J.
通讯作者：
Hausenloy, Derek J.
影响因子：
3.4
作者：
Foadoddini, Mohsen;Esmailidehaj, Mansour;Khoshbaten, Ali
通讯作者：
Khoshbaten, Ali
影响因子：
20.1
作者：
Rassaf, Tienush;Totzeck, Matthias;Kelm, Malte
通讯作者：
Kelm, Malte