Photodynamic therapy of C6-implanted glioma cells in the rat brain employing second-generation photosensitizer talaporfin sodium
Photodynamic therapy of C6-implanted glioma cells in the rat brain employing second-generation photosensitizer talaporfin sodium
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DOI：
10.1016/j.pdpdt.2008.08.001
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发表时间：
2008-09-01
影响因子：
3.3
通讯作者：
Aizawa, Katsuo
中科院分区：
文献类型：
--
作者：
Namatame, Hiroaki;Akimoto, Jiro;Aizawa, Katsuo
对象：光动力学疗法（PDT）作为一种局部治疗恶性胶质瘤的有效性进行了评估，通过调查在大鼠C6胶质瘤模型治疗与他拉泊芬钠，水溶性光敏剂来自叶绿素和曝光的组合的组织学变化的半导体laser.Methods：胶质瘤细胞（C6）在融合期立体定向移植到SD大鼠的右额叶。5天后，大鼠接受右额开颅术并静脉注射他拉泊芬钠。他拉泊芬钠给药后1小时，用664 nm二极管激光束照射每只大鼠。激光照射后1、3、6h分别取脑组织行组织学检查。结果：在未治疗的大鼠中，除了肿瘤块之外，还观察到肿瘤细胞侵入周围水肿的脑组织，范围从脑表面到2mm的深度。PDT后1小时，在0.5mm深度的肿瘤组织中观察到凝固性坏死以及细胞活力的指示消失，例如肿瘤细胞突起消失和细胞质泡沫样变化，伴随着细胞质胶质细胞酸性蛋白（GFAP）表达的减少和颗粒状M30细胞死亡阳性的出现。3小时后，残余肿瘤细胞胞浆中GFAP表达消失，M30细胞死亡表达增加。6 h后，肿胀的肿瘤细胞胞浆呈泡沫状，M30细胞死亡呈强阳性。结论：他拉泊芬钠光动力疗法可诱导大鼠C6胶质瘤凝固性坏死和凋亡。(C)2008 Elsevier B.V.保留所有权利。
Object: The usefulness of photodynamic therapy (PDT) as a local therapy for malignant glioma was evaluated by investigating histological changes in a rat C6 glioma model treated with a combination of talaporfin sodium, a water-soluble photosensitizer derived from chlorophyll and exposure to a diode laser.Methods: Glioma cells (C6) at the confluence stage were transplanted stereotactically into the right frontal lobe of SD rats. Five days later, the rats underwent right frontal craniotomy and intravenous administration of talaporfin sodium. One hour after talaporfin sodium administration, each rat was irradiated by a 664 nm diode laser beam. The brain was removed 1, 3 or 6h after laser irradiation for histological examination of tumor-affected brain tissue and surrounding normal. brain tissue.Results: In addition to the tumor mass, tumor cells invading surrounding edematous brain tissue were seen in untreated rats, ranging from the brain surface to a depth of 2 mm. One hour after PDT, coagulation necrosis as well as disappearance of indication of cell viability such as disappearance of tumor cell processes and foamy changes of cytoplasm were noted in the tumor tissue at a depth of 0.5 mm, accompanied by reduction of cytoplasmic glial fibrillary acidic protein (GFAP) expression and appearance of granular M30 cytodeath positivity. Three hours later, the cytoplasm of the residual tumor cells showed disappearance of GFAP expression and increased expression of M30 cytodeath. Six hours later, the foamy cytoplasm of swollen tumor cells demonstrated strong positivity for M30 cytodeath.Conclusion: PDT using talaporfin sodium induced coagulation necrosis and apoptosis in rats with C6 glioma. (C) 2008 Elsevier B.V. All rights reserved.