Fibroblastic potential of CD41+ cells in the mouse aorta-gonad-mesonephros region and yolk sac.
Fibroblastic potential of CD41+ cells in the mouse aorta-gonad-mesonephros region and yolk sac.
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DOI：
10.1089/scd.2011.0572
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发表时间：
2012-04
影响因子：
4
通讯作者：
Junnian Zhou;Haixu Chen;Si-ting Li;Yi-fan Xie;Wen-yan He;X. Nan;W. Yue;Bing Liu;X. Pei
中科院分区：
文献类型：
--
作者：
Junnian Zhou;Haixu Chen;Si-ting Li;Yi-fan Xie;Wen-yan He;X. Nan;W. Yue;Bing Liu;X. Pei
CD41(αIIb整合素)是巨核细胞和血小板的特异性标志物，最近被发现标志着小鼠胚胎定向造血的启动。然而，胚胎CD41(+)群体是否具有非造血潜能仍然难以捉摸。在此，我们报道了小鼠E11.0腹主动脉-性腺-中肾(AGM)区和卵黄囊(YS)的CD41(+)细胞表达一组间充质标志(逆转录-聚合酶链式反应显示)，在体外间质培养条件下显示出肌成纤维/成纤维细胞的潜能，并在系统移植后在成年受者的肺内分化为α-SMA(+)/表吗啡(+)/波形蛋白(+)细胞。这种具有成纤维细胞潜能的独特细胞群在AGM区域表达中等水平的CD41而不是高水平的CD34。相反，胚胎血流中循环的CD41(+)细胞没有类似的成纤维细胞潜能。与YS相比，AGM来源的CD41(+)细胞具有更强的成纤维细胞潜能，体外生长速度更高。有趣的是，AGM来源的CD41(+)细胞比YS来源的CD41(+)细胞对循环血浆中的趋化因子有更强的反应。我们是第一个在体外和体内展示胚胎CD41(+)群体成纤维细胞潜能的小组，反映了小鼠中期血液和间充质发育之间的密切联系。这些间充质细胞群体的确切来源需要进一步澄清。
CD41 (αIIb integrin), a specific marker for megakaryocytes and platelets, was recently shown to mark the initiation of definitive hematopoiesis in mouse embryos. However, whether embryonic CD41(+) populations have a nonhematopoietic potential remains elusive. Here, we report that the CD41(+) cells from the mouse E11.0 aorta-gonad-mesonephros (AGM) region and yolk sac (YS) expressed a set of mesenchymal markers (as revealed by reverse transcriptase-polymerase chain reaction), displayed myofibroblastic/fibroblastic potential in vitro under mesenchymal culture conditions, and differentiated into α-SMA(+)/epimorphin(+)/vimentin(+) cells in the lungs of adult recipients after systemic transplantation. This unique cell population with fibroblastic potential expressed intermediate rather than high levels of CD41 and was negative for CD34 in the AGM region. In contrast, circulating CD41(+) cells in the embryonic blood stream harbored no similar fibroblastic potential. Compared with the YS, the AGM-derived CD41(+) cells had a more robust fibroblastic potential, as revealed by higher in vitro growth rates. Interestingly, the AGM-derived CD41(+) cells demonstrated a stronger response to the chemotaxin of circulating blood plasma than the YS-derived CD41(+) cells. We are the first group that illustrates the fibroblastic potential of an embryonic CD41(+) population in vitro and in vivo, reflecting the close association between blood and mesenchyme development during mouse mid-gestation. The precise origin of these mesenchymal populations needs further clarification.