A GAL80 Collection To Inhibit GAL4 Transgenes in Drosophila Olfactory Sensory Neurons
A GAL80 Collection To Inhibit GAL4 Transgenes in Drosophila Olfactory Sensory Neurons
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DOI：
10.1534/g3.118.200569
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发表时间：
2018-11-01
影响因子：
2.6
通讯作者：
Matsumura, Ichiro
中科院分区：
文献类型：
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作者：
Eliason, Jessica;Afify, Ali;Matsumura, Ichiro
果蝇识别数百种与生态相关的气味，并对它们做出适当的反应。嗅觉机制的复杂性、冗余性和相互关联性使得确定任何组成神经元或受体对行为的功能贡献的努力变得复杂。一些贡献只能在携带多个突变和转基因的果蝇中阐明，但这种果蝇的生产目前是劳动密集型和耗时的。在这里，我们描述了一组转基因苍蝇，表达酿酒酵母GAL 80在特定的嗅觉感觉神经元（OrX-GAL 80）。GAL 80有效且特异性地减去GAL 4驱动的转基因的活性，所述转基因赋予解剖学和生理学表型。OrX-GAL 80可以让研究人员在无功能的嗅觉背景中有效地激活一种或几种类型的功能神经元。这样的实验将提高我们对气味输入和行为输出之间的机械联系的理解，只有少数功能神经元的分辨率。
Fruit flies recognize hundreds of ecologically relevant odors and respond appropriately to them. The complexity, redundancy and interconnectedness of the olfactory machinery complicate efforts to pinpoint the functional contributions of any component neuron or receptor to behavior. Some contributions can only be elucidated in flies that carry multiple mutations and transgenes, but the production of such flies is currently labor-intensive and time-consuming. Here, we describe a set of transgenic flies that express the Saccharomyces cerevisiae GAL80 in specific olfactory sensory neurons (OrX-GAL80s). The GAL80s effectively and specifically subtract the activities of GAL4-driven transgenes that impart anatomical and physiological phenotypes. OrX-GAL80s can allow researchers to efficiently activate only one or a few types of functional neurons in an otherwise nonfunctional olfactory background. Such experiments will improve our understanding of the mechanistic connections between odorant inputs and behavioral outputs at the resolution of only a few functional neurons.