Infertility resulting from transgenic I-PpoI male Anopheles gambiae in large cage trials
Infertility resulting from transgenic I-PpoI male Anopheles gambiae in large cage trials
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DOI：
10.1179/2047773212y.0000000003
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发表时间：
2012-03-01
影响因子：
3.4
通讯作者：
Benedict, M. Q.
中科院分区：
文献类型：
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作者：
Klein, T. A.;Windbichler, N.;Benedict, M. Q.
目的：冈比亚按蚊是撒哈拉以南非洲地区疟疾的主要传播媒介，是遗传控制计划的潜在目标。我们测定了男性A的能力。冈比亚通过生殖系转化将不育引入稳定的年龄分布人口。我们还确定了转基因对生活史的影响。冈比亚蚊子在大的室内笼中饲养。携带I-PpoI归巢核酸内切酶基因的雄性蚊子以65和610的释放速率引入，其中它们与目标雄性蚊子竞争交配。类似的试验进行了一个额外的x 1释放level.Results的小笼子里：不孕症被成功地引入到所有的目标人群。在支持性实验中，在所有的A. gambiae复合体，转基因雄性与之交配。生活史实验表明，I-PpoI雄性活力的降低以减少成年雄性的出现、寿命和竞争力的形式存在。冈比亚I-PpoI雄性能够在室内笼试验中在目标群体中引入高水平的不育。尽管HEG转基因导致活力损失，但这还是实现了。这些结果激发了对性I-PpoIA的进一步试验。冈比亚在室外笼和田间试验。
Objectives: Anopheles gambiae is the primary vector of malaria in sub-Saharan Africa and is a potential target of genetic control programs. We determined the capacity of male A. gambiae created by germline transformation to introduce infertility into stable age-distribution populations. We also determined effects of the transgenes on life history.Methods: Stable age-distribution populations of A. gambiae mosquitoes were established in large indoor cages. Male mosquitoes carrying an I-PpoI homing endonuclease gene were introduced at 65 and 610 release rates where they competed with target male mosquitoes for matings. Similar trials were conducted in small cages with an additional x 1 release level.Results: Infertility was successfully introduced into all target populations. In supporting experiments, complete female infertility was observed in all strains and species of the A. gambiae complex to which transgenic males were mated. Life history experiments demonstrated that reductions in I-PpoI male vigor exist in the form of reduced adult male emergence, longevity and competitiveness.Discussion: A. gambiae I-PpoI males are capable of introducing high levels of infertility in target populations in indoor cage trials. This was accomplished despite losses of vigor resulting from the HEG transgene. These results motivate further trials of sexually I-PpoI A. gambiae in outdoor cage and field trials.