Spatial variability of hydrolytic and oxidative potential enzyme activities in different subsoil compartments
Spatial variability of hydrolytic and oxidative potential enzyme activities in different subsoil compartments
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DOI：
10.1007/s00374-015-0992-5
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发表时间：
2015-01
影响因子：
6.5
通讯作者：
M. Uksa;M. Schloter;T. Kautz;Miriam Athmann;U. Köpke;D. Fischer
中科院分区：
文献类型：
--
作者：
M. Uksa;M. Schloter;T. Kautz;Miriam Athmann;U. Köpke;D. Fischer
过去很少有人研究底土养分周转的空间异质性，尽管旱层和根际被发现是这种少营养环境中重要的微生物热点。在本研究中，我们测定了不同土室底土和表土中不同潜在酶的活性。结果表明，无论是表层土壤还是底层土壤，水解酶的活性都显著高于旱层和根际样品，水解酶能裂解有效的有机底物。在土壤中，水解酶活性随深度的增加而降低。相反，参与顽固有机物质腐烂的氧化酶从微生物群落中释放出来，特别是在底土的大部分中。这强调了底土对养分获取的重要性，并为具有不同生活方式的微生物提供了明显的空间分离证据。
The spatial heterogeneity of nutrient turnover in subsoils has been rarely studied in the past, although drilosphere and rhizosphere are found to be important microbial hotspots in this oligotrophic environment. In this study, we measured different potential enzyme activities in different soil compartments of subsoil and topsoil. It could be shown that the activities of hydrolases, which cleave readily available organic substrates, are significantly higher in samples from the drilosphere and rhizosphere both in topsoil and subsoil. In bulk soil, hydrolase activities decrease with depth. In contrast, oxidative enzymes, which are involved in the decay of recalcitrant organic material, are released from the microbial community especially in the bulk fraction of subsoil. This emphasizes the importance of subsoil for nutrient acquisition and gives evidence for a distinct spatial separation of microbes with diverging lifestyles.