A comparative analysis of intraperitoneal versus intracerebroventricular administration of bromodeoxyuridine for the study of cell proliferation in the adult rat brain
A comparative analysis of intraperitoneal versus intracerebroventricular administration of bromodeoxyuridine for the study of cell proliferation in the adult rat brain
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DOI：
10.1016/j.jneumeth.2011.08.006
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发表时间：
2011-10-15
影响因子：
3
通讯作者：
Fernandez-Llebrez, P.
中科院分区：
文献类型：
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作者：
Cifuentes, M.;Perez-Martin, M.;Fernandez-Llebrez, P.
溴脱氧尿苷 (BrdU) 是检测成人大脑中增殖细胞最广泛使用的标记物。在这里，我们分析示踪剂的给药途径是否影响标记细胞的数量。对于 BrdU 的腹腔内 (ip) 给药，我们在 7 天内每天注射两次，而对于脑室内 (icy) 给药，我们也在 7 天内将其连续注入一侧脑室。腹膜内给药后，在侧脑室纹状体壁的室下区、海马和脑室周围神经血管器官中可见BrdU标记的细胞。此外，缰核和大的有髓束，例如穹窿和胼胝体，显示出许多 BrdU 阳性细胞核。大脑其余部分的实质区域中标记的细胞核很少。相比之下，在冰冷施用标记物后，在脑室周围区的实质中观察到 BrdU 阳性细胞核的数量显着增加，从而表明当将示踪剂直接施用到脑室脑脊液中时，示踪剂的可用性更高。我们认为，增殖细胞附近 BrdU 的可用性可能取决于脑血管对每个位置核苷的渗透性。通过使用双重免疫细胞化学，我们发现神经元、星形胶质细胞、少突胶质细胞、单胞细胞和小胶质细胞掺入了示踪剂，证明了它们的增殖能力。 (C) 2011 Elsevier B.V. 保留所有权利。
Bromodeoxyuridine (BrdU) is the most widely used marker to detect proliferative cells in the adult brain. Here we analyse whether the route of administration of the tracer influences the number of labelled cells. For the intraperitoneal (ip) administration of BrdU, we performed two daily injections during 7 days, and for an intracerebroventricular (icy) delivery, it was continuously infused into one lateral ventricle for a 7 days period as well. After ip administration, cells labelled with BrdU were seen in the subventricular zone of the striatal wall of the lateral ventricle, the hippocampus and the neurohemal circumventricular organs. Also, the habenula and large myelinated tracts, such as the fornix and the corpus callosum, showed many BrdU-positive nuclei. Labelled nuclei were scarce in the parenchymal regions of the rest of the brain. In contrast, a significant increase in the number of BrdU-positive nuclei was observed in the parenchyma of the periventricular zones after icy administration of the marker, thus showing a greater availability of the tracer when it was administered directly into the ventricular cerebrospinal fluid. We suggest that the availability of BrdU in the vicinity of proliferating cells may depend on the permeability of the brain vessels to nucleosides in each location. By using double immunocytochemistry we found that neurons, astrocytes, oligodendrocytes, tanycytes and microglia had incorporated the tracer, demonstrating their proliferation capacity. (C) 2011 Elsevier B.V. All rights reserved.