Chemical Groups and Structural Characterization of Brown-Rotted Pinus massoniana Lignin
Chemical Groups and Structural Characterization of Brown-Rotted Pinus massoniana Lignin
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DOI：
10.1080/10236660802586459
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发表时间：
2009-01
影响因子：
1.9
通讯作者：
Qining Sun;T. Qin;Gai‐Yun Li
中科院分区：
文献类型：
--
作者：
Qining Sun;T. Qin;Gai‐Yun Li
摘要 通过 Björkman 和 Lundquist 技术，从完好且自然褐腐的马尾松（由褐腐真菌茯苓腐烂）中分离出磨碎的木质素（MWL）。通过 FT-IR、UV、1 H NMR、GPC、ESR 光谱和化学分析方法对两种木质素进行了表征。结果表明，与完好木质素相比，褐腐木质素的总酸性基团、羰基、羧基和酚羟基含量较高，但甲氧基含量较低。完好木质素和褐腐木质素的 C9 公式分别计算为 C9H8.78O2.85(OCH3)0.83 和 C9H8.63O3.01(OCH3)0.71。完好木质素每个C9单元含有0.75个脂肪族羟基和0.17个酚性羟基，褐腐木质素每个C9单元含有0.62个脂肪族羟基和0.30个酚性羟基。木质素主要为愈创木基型木质素，β-O-4结构构成主要单体间连接。根据分析结果和提出的反应机理，脱甲基化、氧化和解聚是褐腐真菌对木质素最重要的修饰。
Abstract Milled wood lignins (MWLs) were isolated from sound and naturally brown-rotted Pinus massoniana (decayed by brown-rot fungus Poria cocos) by Björkman and Lundquist techniques. Both lignins were characterized by FT-IR, UV, 1 H NMR, GPC, ESR spectroscopy, and chemical analyses methods. The results indicated the brown-rotted lignin was higher in the total acidic group, carbonyl, carboxyl, and phenol hydroxyl contents, but lower in methoxyl content than sound lignin. The C9 formulas were calculated for sound and brown-rotted lignin as C9H8.78O2.85(OCH3)0.83 and C9H8.63O3.01(OCH3)0.71, respectively. Sound lignin had 0.75 aliphatic hydroxyl group and 0.17 phenolic hydroxyl group per C9-unit, and brown-rotted lignin had 0.62 aliphatic hydroxyl group and 0.30 phenolic hydroxyl group per C9-unit; lignins were mainly guaiacyl-type lignin, and β-O-4 structures constituted the main intermonomeric connections. Based on the analytical results and proposed reaction mechanism, demethylation, oxidation, and depolymerization were the most important modifications of lignin by brown-rotted fungi.