Glutathione transferase activity and expression patterns during grain filling in flag leaves of wheat genotypes differing in drought tolerance: Response to water deficit
Glutathione transferase activity and expression patterns during grain filling in flag leaves of wheat genotypes differing in drought tolerance: Response to water deficit
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DOI：
10.1016/j.jplph.2009.05.016
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Erdei, Laszlo
中科院分区：
文献类型：
--
作者：
Galle, Agnes;Csiszar, Jolan;Erdei, Laszlo
用分光光度法测定了小麦品种总谷胱甘肽S转移酶(GST，EC 2.5.1.18)和谷胱甘肽过氧化物酶(GPOX)活性。MV Emese和cv.Plainsman(耐旱性)和cv.GK Elet和Cappelle Desprez(干旱敏感)旗叶在灌浆期的对照和干旱胁迫条件下，以揭示不同GST类物质在旗叶衰老过程中可能的作用。以3个GST类的6个小麦GST为材料，研究了干旱和衰老对GST的调控作用。耐旱品种普莱斯曼的GPOX活性(EC 1.11.1.9)在水分充足的对照中表现较高。同时，对TaGSTU1B和TaGSTF6序列进行实时定量聚合酶链式反应分析，结果表明，TaGSTU1B和TaGSTF6基因的高表达水平随时间的延长而增加，表明这些基因的基因产物可能在小麦单果衰老过程中发挥重要作用。干旱胁迫也诱导了这些基因在4个供试品种中的表达，但cv.普莱斯曼。我们的数据显示了小麦GSTs的基因变异。干旱条件下两个与衰老相关的GSTs在旗叶中的表达水平和早期诱导与产量稳定性相关。(C)2009年爱思唯尔股份有限公司。版权所有。
Total glutathione S-transferase (GST, EC 2.5.1.18) and glutathione peroxidase (GPOX) activity were measured spectrophotometrically in Triticum aestivum cv. MV Emese and cv. Plainsman (drought tolerant) and cv. GK Elet and Cappelle Desprez (drought-sensitive) flag leaves under control and drought stress conditions during the grain-filling period, in order to reveal possible rotes of different GST classes in the senescence of flag leaves. Six wheat GSTs, members of 3 GST classes, were selected and their regulation by drought and senescence was investigated. High GPOX activity (EC 1.11.1.9) was observed in well-watered controls of the drought-tolerant Plainsman cultivar. At the same time, TaGSTU1B and TaGSTF6 sequences, investigated by real-time PCR, showed high-expression levels that increased with time, indicating that the gene products of these genes may play important roles in monocarpic senescence of wheat. Expression of these genes was also induced by drought stress in all of the four investigated cultivars, but extremely high transcript amounts were detected in cv. Plainsman. Our data indicate genotypic variations of wheat GSTs. Expression levels and early induction of two senescence-associated GSTs under drought during grain fitting in flag leaves correlated with high yield stability. (C) 2009 Elsevier GmbH. All rights reserved.