Comparison of cold hardiness and sugar content between diapausing and nondiapausing pupae of the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)
Comparison of cold hardiness and sugar content between diapausing and nondiapausing pupae of the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)
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DOI：
10.1111/j.0307-6962.2005.00422.x
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发表时间：
2005-03-01
影响因子：
1.5
通讯作者：
Tsumuki, H
中科院分区：
文献类型：
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作者：
Izumi, Y;Anniwaer, K;Tsumuki, H
为了解棉铃虫的越冬性，比较了滞育蛹与非滞育蛹的抗寒性和糖含量。将在20 ℃短和长光周期下饲养的滞育和非滞育的纯种马驯化，每5天降低5 ℃至0 ℃。当驯化温度达到0 ℃时，评估滞育蛹的存活。随着治疗期的进展，存活率逐渐降低，大约一半存活112天。然而，非滞育蛹暴露于0 ℃后仅存活14天。非滞育、滞育和驯化蛹的过冷却点约为-17 ℃。腐殖酸中含有的主要糖是海藻糖和葡萄糖。尽管滞育蛹中的海藻糖含量（初始水平：0.6 mg/100 mg（-1）鲜重）在冷驯化期间显著增加，并且持续增加直到暴露于0 ℃后58天（最高水平：1.8 mg/100 mg（-1）），但葡萄糖在0 ℃下保持在低水平（0.02 mg/100 mg（-1））达56天。然而，暴露于0 ℃后84天，葡萄糖含量增加（最高水平：0.8 mg 100 mg（-1）），海藻糖含量降低。冷驯化过程中糖原含量逐渐降低。当非滞育蛹从化蛹开始到眼斑移动，每5天降低5 ℃至5 ℃进行驯化时，海藻糖含量增加（最高水平：1.0 mg 100 mg（-1））。非滞育蛹的葡萄糖含量在羽化前增加（0.09mg ~ 100 mg ~（-1））。结果表明，H.棉铃虫能在冬季平均气温高于0 ℃的地区越冬，而非滞育性棉铃虫则不能。
To understand overwintering of the cotton boll worm Helicoverpa armigera, cold hardiness and sugar content are compared between diapausing and nondiapausing pupae. Diapausing and nondiapausing pupae reared at 20 degreesC under short and long photoperiods are acclimatized with a reduction of 5 degreesC per 5 days to 0 degreesC. When the acclimation temperature reaches 0 degreesC, the survival of diapausing pupae is assessed. The survival gradually decreases as the period of treatment progresses and approximately half survive for 112 days. However, nondiapausing pupae survive only 14 days after exposure to 0 degreesC. The surpercooling points of nondiapausing, diapausing and acclimatized pupae are approximately -17 degreesC. The major sugars contained in pupae are trehalose and glucose. Even though trehalose contents in diapausing pupae (initial level: 0.6 mg 100 mg(-1) fresh weight) increase significantly during cold acclimation and continue increasing until 58 days after exposure to 0 degreesC (maximum level: 1.8 mg 100 mg(-1)), glucose is maintained at low levels (0.02 mg 100 mg(-1)) for 56 days at 0 degreesC. However, glucose contents increase (maximum level: 0.8 mg 100 mg(-1)) with decreasing contents of trehalose 84 days after exposure to 0 degreesC. Glycogen content gradually decreases during cold acclimation. When nondiapausing pupae are acclimatized with a reduction of 5 degreesC per 5 days to 5 degreesC from the beginning of pupation until the eyespots move, trehalose content increases (maximum level: 1.0 mg 100 mg(-1)). Glucose contents in nondiapausing pupae increase before eclosion (0.09 mg 100 mg(-1)). From these results, diapausing pupae of H. armigera can overwinter in regions where average winter temperatures are higher than 0 degreesC, but nondiapausing pupae cannot.