Enhanced plasmid stability and production of hEGF by immobilized recombinant E-coli JM101
Enhanced plasmid stability and production of hEGF by immobilized recombinant E-coli JM101
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DOI：
10.1016/j.bej.2005.03.005
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发表时间：
2006-03-01
影响因子：
3.9
通讯作者：
Wong, WKR
中科院分区：
文献类型：
--
作者：
Chen, XA;Xu, ZN;Wong, WKR
以多孔聚氨酯泡沫（PUF）为载体，固定化重组大肠杆菌JM 101，用于人表皮生长因子（hEGF）的生产。摇瓶培养结果表明，固定化细胞在PUF中可以促进细胞生长和hEGF表达。分别采用自行设计的鼓泡塔和三相流化床生物反应器进行hEGF的生产。结果表明，聚氨酯泡沫是一种可行的固定化载体材料，具有良好的生物相容性。固定化还可以降低分离质粒丢失和无质粒细胞过度生长的可能性。细胞密度、质粒稳定性和hEGF产量分别高于无泡沫基质的那些。hEGF产量从游离培养的8.73mg/l提高到固定化培养的11.4mg/l h。(c)2006年由Elsevier B. V.出版
Recombinant Escherichia coli JM101 was immobilized with porous polyurethane foam (PUF) particle as supporter matrix for human epidermal growth factor (hEGF) production. Flask culture showed that cell immobilization in PUF can improve cell growth and hEGF expression. A bubble column and a three-phase fluidized bed bioreactor by self-design was further applied to produce hEGF, respectively. The results demonstrated that PUF is a feasible immobilized supporter material with good biocompatibility. Immobilization could also decrease the probability for segregational plasmid loss and overgrowth of plasmid-free cells. Cell density, plasmid stability and hEGF productivity were higher than those without the foam matrix, respectively. hEGF productivity was enhanced from 8.73 mg/l It of free-culture to 11.4 mg/l h of immobilized cultivation. (c) 2006 Published by Elsevier B.V.