Gene expression patterns in visual cortex during the critical period: Synaptic stabilization and reversal by visual deprivation
Gene expression patterns in visual cortex during the critical period: Synaptic stabilization and reversal by visual deprivation
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DOI：
10.1073/pnas.0710172105
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发表时间：
2008-07-08
影响因子：
11.1
通讯作者：
Sur, Mriganka
中科院分区：
文献类型：
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作者：
Lyckman, Alvin W.;Horng, Sam;Sur, Mriganka
眼特异性的，geniculocortical输入到初级视觉皮层（V1）的映射是高度敏感的双眼之间的相关活动的平衡在有限的出生后关键时期的眼优势可塑性。在这个关键时期，调节突触可塑性的基因和蛋白质的表达可能会增加。在小鼠V1转录的DNA微阵列分析之前，期间，和之后的关键时期确定了31个基因的上调和22个在关键时期下调。排名最高的上调基因心脏肌钙蛋白C编码一种神经元钙结合蛋白，该蛋白调节肌动蛋白结合，其表达具有活性依赖性，并且对第4层星星锥体神经元具有相对选择性。下调程度最高的基因synCAM也具有基于肌动蛋白的功能。肌动蛋白结合功能，G蛋白信号转导，转录和髓鞘形成的关键时期转录组显着代表。关键期单眼剥夺可逆转几乎所有关键期基因的表达。调控基因的概况表明，突触稳定性是关键期基因表达的主要驱动因素，视觉活动的改变驱动稳定性的稳态恢复。
The mapping of eye-specific, geniculocortical inputs to primary visual cortex (V1) is highly sensitive to the balance of correlated activity between the two eyes during a restricted postnatal critical period for ocular dominance plasticity. This critical period is likely to have amplified expression of genes and proteins that mediate synaptic plasticity. DNA microarray analysis of transcription in mouse V1 before, during, and after the critical period identified 31 genes that were up-regulated and 22 that were down-regulated during the critical period. The highest-ranked up-regulated gene, cardiac troponin C, codes for a neuronal calcium-binding protein that regulates actin binding and whose expression is activity-dependent and relatively selective for layer-4 star pyramidal neurons. The highest-ranked down-regulated gene, synCAM, also has actin-based function. Actin-binding function, G protein signaling, transcription, and myelination are prominently represented in the critical period transcriptome. Monocular deprivation during the critical period reverses the expression of nearly all critical period genes. The profile of regulated genes suggests that synaptic stability is a principle driver of critical period gene expression and that alteration in visual activity drives homeostatic restoration of stability.