Exploring tomato Solanum pennellii introgression lines for residual biomass and enzymatic digestibility traits.
Exploring tomato Solanum pennellii introgression lines for residual biomass and enzymatic digestibility traits.
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DOI：
10.1186/s12863-016-0362-9
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发表时间：
2016-04-05
期刊：
影响因子：
2.9
通讯作者：
Ercolano MR
中科院分区：
文献类型：
--
作者：
Caruso G;Gomez LD;Ferriello F;Andolfi A;Borgonuovo C;Evidente A;Simister R;McQueen-Mason SJ;Carputo D;Frusciante L;Ercolano MR
用于燃料转换的剩余生物质生产是一个独特的机会，可以避免对使用专用原料作物影响粮食供应的担忧。开发既适合食物消费又适合燃料转换的番茄品种，需要建立新的选择方法。对番茄茄子导入群体的果实产量、生物量表型多样性和糖化潜力进行了研究。导入系2-5、2-6、6-3、7-2、10-2和12-4是果实产量和剩余生物量的最佳组合。木质素、纤维素、半纤维素含量和糖化率在供试品系间差异较大。在半纤维素中，IL 6-3、IL 7-2和IL 6-2的木糖值较高，而IL 10-2、IL 6-3和IL 2-6的阿拉伯糖含量较低。大部分供试品系的糖化值均以碱预处理最高，表明化学预处理是改善生物质加工性能的重要因素。有趣的是，不止一个性状的极端基因类型被发现，这使得更好的基因类型得以鉴定。与番茄生物产量和消化率变异相关的基因组区域的细胞壁相关基因定位突出了潜在的候选基因。它们中的少数几个的分子表达谱提供了关于挑战途径的有用信息。彭尼利渐渗群体的筛选对于深入研究生物量生产和消化率等复杂性状是非常有用的。鉴定出的极端基因类型可以有效地用于遗传研究和育种。本文的在线版本(doi：10.1186/s12863-0160362-9)包含补充材料，授权用户可以使用。
Residual biomass production for fuel conversion represents a unique opportunity to avoid concerns about compromising food supply by using dedicated feedstock crops. Developing tomato varieties suitable for both food consumption and fuel conversion requires the establishment of new selection methods. A tomato Solanum pennellii introgression population was assessed for fruit yield, biomass phenotypic diversity, and for saccharification potential. Introgression lines 2–5, 2–6, 6–3, 7–2, 10–2 and 12–4 showed the best combination of fruit and residual biomass production. Lignin, cellulose, hemicellulose content and saccharification rate showed a wide variation in the tested lines. Within hemicellulose, xylose value was high in IL 6–3, IL 7–2 and IL 6–2, whereas arabinose showed a low content in IL 10–2, IL 6–3 and IL 2–6. The latter line showed also the highest ethanol potential production. Alkali pre-treatment resulted in the highest values of saccharification in most of lines tested, suggesting that chemical pretreatment is an important factor for improving biomass processability. Interestingly, extreme genotypes for more than one single trait were found, allowing the identification of better genotypes. Cell wall related genes mapping in genomic regions involved into tomato biomass production and digestibility variation highlighted potential candidate genes. Molecular expression profile of few of them provided useful information about challenged pathways. The screening of S. pennellii introgression population resulted very useful for delving into complex traits such as biomass production and digestibility. The extreme genotypes identified could be fruitfully employed for both genetic studies and breeding. The online version of this article (doi:10.1186/s12863-016-0362-9) contains supplementary material, which is available to authorized users.