THE CHEMICAL NATURE OF THE ACROSOME IN THE MALE GERM CELLS
THE CHEMICAL NATURE OF THE ACROSOME IN THE MALE GERM CELLS
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DOI：
10.1073/pnas.36.11.677
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发表时间：
1950-01-01
影响因子：
11.1
通讯作者：
SCHRADER, F
中科院分区：
文献类型：
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作者：
LEUCHTENBERGER, C;SCHRADER, F
动物精子顶体与高尔基体的紧密联系最早是由鲍文在1923年提出的，他认为顶体物质可能是从高尔基体中“分泌”出来的。虽然他的细胞学研究似乎支持顶体来自高尔基体的概念，但所用的染色方法不允许对这两种结构的化学性质做出任何结论。最近细胞化学程序的发展，利用特定的染色反应对细胞成分进行原位化学表征，现在使分析雄性生殖细胞的一些化学成分成为可能。白纹伊蚊是一种半翅目昆虫，由于其精母细胞、精子细胞和精子的大小存在显著差异，因此为细胞化学研究提供了特别有利的材料。2、19、20本文提出的证据表明，精子顶体来源于初级精母细胞的腺体，并且都含有1，2-乙二醇基的多糖。在第三和第五叶的大细胞中，精母细胞、精母细胞和精子的顶体中的多糖含量显著高于其余四叶的正常细胞和小细胞。为了鉴定睾丸细胞中的多糖，我们使用了微量高碘酸席夫(PAS)反应，导致组织中的多糖结构染色，如1948年Hotchkiss5所描述的那样。根据Hotchkiss5和McManus的说法，12当以1，2乙二醇基存在时，高碘酸与碳水化合物的反应被认为如下：
The close connection of the acrosome of the animal sperm with the Golgi apparatus was first suggested by Bowen'in 1923, who stated that the acrosomal material might be" secreted" from the Golgi apparatus. While his cytological studies seem to support the concept that the acrosome is derived from the Golgi apparatus, the staining methods employed do not allow any conclusions as to the chemical nature of either structure. The recent development of cytochemical procedures, using specific staining reactions for the chemical characterization of cellular constituents in situ now makes possible an analysis of some of the chemical components of the dictyosomal material22 and the acrosome of the male germcells. Arvelius albopunctatus, an hemipteran insect, provides an especially favorable material for the cytochemical studies, since the different lobes of the testis are characterized by a constant and marked difference in the size of their spermatocytes, spermatids and sperms. 2 19, 20 In the present paper evidence is presented that theacrosome of thesperm is derived from the dictyosomes of the primary spermatocytes, and that both contain poly-saccharides in a 1, 2 glycol grouping. The amount ofpolysaccharides in the dictyosomal material and in the acrosome of spermatocytes, spermatids and sperms is strikingly higher in the large sized cells of the third and fifth lobes than in the normal and small sized cells of the remaining four lobes. For the identification of the polysaccharides in the cells of the testis we used the microchemical periodic acid Schiff (PAS) reaction resulting in the staining of polysaccharide structures in tissues as described by Hotchkiss5 in 1948. According to Hotchkiss5 and McManus, 12 the reaction of periodic acid with carbohydrates, when present as 1, 2 glycol grouping, is considered to be the following: