Nucleotide sequence of pea cDNA encoding chloroplast carbonic anhydrase.
Nucleotide sequence of pea cDNA encoding chloroplast carbonic anhydrase.
复制标题
编码叶绿体碳酸酐酶的豌豆 cDNA 的核苷酸序列。
DOI：
10.1093/nar/18.11.3413
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发表时间：
1990
影响因子：
14.9
通讯作者：
W. Ogren
中科院分区：
文献类型：
--
作者：
C. Roeske;W. Ogren
从豌豆（Pisum sativum L.）用抗菠菜碳酸酐酶（EC 4.2. 1.1）。984 bp的开放阅读框包含了成熟叶绿体蛋白及其转运肽的完整编码区。该蛋白的同一性通过与菠菜碳酸酐酶的强同源性来验证（1）。与哺乳动物同工酶几乎没有序列同源性（2）。基于成熟多肽的Edman降解，转运肽是104个氨基酸。这比通常发现的核编码叶绿体蛋白质更长（3）。即使对于富含丝氨酸和茶碱的叶绿体转运肽，也不寻常的是由Thr残基（氨基酸残基36-43）限定的七个连续Ser残基的区域。推导的224个氨基酸的成熟叶绿体多肽的分子量为24.2kd。
A 1.3 kb cDNA was isolated from a pea (Pisum sativum L.) cDNA library by immunoscreening with rabbit antiserum to spinach carbonic anhydrase (EC 4.2. 1.1). The 984 bp open reading frame contained the entire coding region of the mature chloroplast protein and its transit peptide. The identity of the protein is verified by the strong homology with carbonic anhydrase from spinach (1). There is little sequence homology with mammalian isozymes (2). The transit peptide, based on Edman degradation of the mature polypeptide, is 104 amino acids. This is longer than commonly found for nuclear-encoded chloroplast proteins (3). Also unusual, even for the serine-and theonine-rich chloroplast transit peptides, is a region of seven consecutive Ser residues bounded by Thr residues (amino acid residues 36-43). The molecular mass of the deduced 224 amino acid mature chloroplast polypeptide is 24.2 kd.