Bile Acid Is a Host Factor That Regulates the Composition of the Cecal Microbiota in Rats
Bile Acid Is a Host Factor That Regulates the Composition of the Cecal Microbiota in Rats
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DOI：
10.1053/j.gastro.2011.07.046
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发表时间：
2011-11-01
期刊：
影响因子：
29.4
通讯作者：
Yokota, Atsushi
中科院分区：
文献类型：
--
作者：
Islam, K. B. M. Saiful;Fukiya, Satoru;Yokota, Atsushi
背景与目的：胃肠道菌群的改变与代谢性疾病有关。然而，很少有人知道宿主因素，诱导胃肠道细菌种群的变化。我们研究了胆汁酸在这一过程中的作用，因为它们具有很强的抗菌活性，特别是胆酸给药对大鼠模型肠道微生物群组成的影响。方法：用添加不同浓度胆酸的饲料喂养大鼠10天。我们使用16 S核糖体RNA基因克隆文库测序和荧光原位杂交来表征不同饮食组的盲肠微生物群的组成。还分析了粪便中的胆汁酸、盲肠内容物中的有机酸和一些血液参数。研究结果：胆酸的管理诱导门水平的肠道微生物群的组成的变化;厚壁菌门占主导地位的拟杆菌。胆酸喂养简化了微生物群的组成，并伴随着梭菌和丹毒丝菌类中的几种细菌的生长。外部给药的胆酸通过细菌7 α-脱羟基反应有效转化为脱氧胆酸。给予胆酸饮食的大鼠血清脂联素水平显著降低。结论：胆酸调节大鼠肠道微生物群的组成，引起与高脂饮食引起的变化相似的变化。这些发现提高了我们对代谢疾病与胃肠道微生物群组成之间关系的理解。
BACKGROUND & AIMS: Alterations in the gastrointestinal microbiota have been associated with metabolic diseases. However, little is known about host factors that induce changes in gastrointestinal bacterial populations. We investigated the role of bile acids in this process because of their strong antimicrobial activities, specifically the effects of cholic acid administration on the composition of the gut microbiota in a rat model. METHODS: Rats were fed diets supplemented with different concentrations of cholic acid for 10 days. We used 16S ribosomal RNA gene clone library sequencing and fluorescence in situ hybridization to characterize the composition of the cecal microbiota of the different diet groups. Bile acids in feces, organic acids in cecal contents, and some blood parameters were also analyzed. RESULTS: Administration of cholic acid induced phylum-level alterations in the composition of the gut microbiota; Firmicutes predominated at the expense of Bacteroidetes. Cholic acid feeding simplified the composition of the microbiota, with outgrowth of several bacteria in the classes Clostridia and Erysipelotrichi. Externally administered cholic acid was efficiently transformed into deoxycholic acid by a bacterial 7 alpha-dehydroxylation reaction. Serum levels of adiponectin decreased significantly in rats given the cholic acid diet. CONCLUSIONS: Cholic acid regulates the composition of gut microbiota in rats, inducing similar changes to those induced by high-fat diets. These findings improve our understanding of the relationship between metabolic diseases and the composition of the gastrointestinal microbiota.