Direct interaction of resistance gene and avirulence gene products confers rice blast resistance
Direct interaction of resistance gene and avirulence gene products confers rice blast resistance
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DOI：
10.1093/emboj/19.15.4004
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发表时间：
2000-08-01
期刊：
影响因子：
11.4
通讯作者：
Valent, B
中科院分区：
文献类型：
--
作者：
Jia, Y;McAdams, SA;Valent, B
表达Pi-To基因的水稻对水稻爆炸真菌的菌株具有抗性，在基因 - 基因关系中表达AVR-PITA。 PI-TA用C-末端编码具有中央局部核苷酸结合位点和富含亮氨酸的结构域（LRD）的假定细胞质受体。预计AVR-PITA将用N末端分泌信号和蛋白序列编码金属蛋白酶。 AVR-PITA（176）缺乏分泌和蛋白质序列。我们在这里报告说，植物细胞内AVR-PITA（176）的瞬时表达会导致Pi-Ta依赖性抗性反应，AVR-PITA（176）蛋白在酵母菌两性杂交系统和体外结合测定中都与Pi-Ta蛋白的LRD特别结合。 Pi-Ta LRD或AVR-PITA（176）蛋白酶基序中的单个氨基酸取代，导致植物中耐药性丧失也破坏了酵母和体外的物理相互作用。这些数据表明，AVR-PITA（176）蛋白直接与植物细胞内的Pi-Ta LRD区域结合，以启动Pi-TN介导的防御反应。
Rice expressing the Pi-to gene is resistant to strains of the rice blast fungus, Magnaporthe grisea, expressing AVR-Pita in a gene-for-gene relationship. Pi-ta encodes a putative cytoplasmic receptor with a centrally localized nucleotide-binding site and leucine-rich domain (LRD) at the C-terminus. AVR-Pita is predicted to encode a metalloprotease with an N-terminal secretory signal and pro-protein sequences. AVR-Pita(176) lacks the secretory and pro-protein sequences. We report here that transient expression of AVR-Pita(176) inside plant cells results in a Pi-ta-dependent resistance response, AVR-Pita(176) protein is shown to bind specifically to the LRD of the Pi-ta protein, both in the yeast two-hybrid system and in an in vitro binding assay. Single amino acid substitutions in the Pi-ta LRD or in the AVR-Pita(176) protease motif that result in loss of resistance in the plant also disrupt the physical interaction, both in yeast and in vitro. These data suggest that the AVR-Pita(176) protein binds directly to the Pi-ta LRD region inside the plant cell to initiate a Pi-tn-mediated defense response.