Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain
Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain
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DOI：
10.1016/j.brainres.2010.03.056
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发表时间：
2010-05-17
期刊：
影响因子：
2.9
通讯作者：
Hay-Schmidt, Anders
中科院分区：
文献类型：
--
作者：
Hundahl, Christian Ansgar;Allen, Gregg C.;Hay-Schmidt, Anders
本研究旨在利用原位杂交、免疫组织化学和免疫电镜技术表征细胞红蛋白（Cygb）和神经红蛋白（Ngb）在小鼠脑中的解剖和亚细胞定位。Cygb和Ngb只存在于不同的大脑区域，而且经常出现在相同的区域。我们在梨状皮质、杏仁核、下丘脑(内侧视前区、交叉上核、下丘脑外侧（LH）、下丘脑腹内侧核、弓形核、束核、外侧背侧被盖核、桥脚被盖核、蓝斑核、孤束核和三叉脊髓核中发现了强烈的染色。此外，Cygb还存在于海马、丘脑网状核和中缝背核中；Ngb位于臂旁核下。Cygb和Ngb的共定位主要见于LDTg和PPTg。免疫电镜观察发现Cygb和Ngb分布于细胞质、沿神经小管、线粒体和细胞核中。大多数神经元一氧化氮合酶（nNOS）阳性的神经元与Cygb共定位，尽管并非所有nNOS神经元都含有Cygb。Ngb与LH中几乎所有的促食欲素神经元共定位。总之，Cygb和Ngb的分布似乎比以前报道的更受限制和连贯。我们认为应该考虑除纯氧缓冲剂和神经保护剂以外的其他功能。解剖数据表明，Cygb参与NO信号传导，并参与Cygb和Ngb的睡眠-觉醒循环。(C) 2010 Elsevier B.V.版权所有
The present study aimed at characterizing the anatomical and subcellular localization of cytoglobin (Cygb) and neuroglobin (Ngb) in the mouse brain by use of in situ hybridisation, immunohistochemistry and immunoelectron microscopy. Cygb and Ngb were only found in distinct brain areas and often in the same areas. We found intense staining in the piriform cortex, amygdala, hypothalamus (medial preoptic area, supra chiasmatic nucleus, lateral hypothalamus (LH), ventromedial hypothalamic nucleus, and the arcuate nucleus, habenular nuclei, laterodorsal tegmental nucleus (LDTg), pedunculopontine tegmental nucleus (PPTg), locus coeruleus, nucleus of the solitary tract and the spinal trigeminal nucleus. In addition Cygb is found in the hippocampus, the reticular thalamic nucleus, and the dorsal raphe nucleus; Ngb is found in the sub parabrachial nucleus. Co-localization of Cygb and Ngb is mainly observed in the LDTg and PPTg. Cygb and Ngb were found in cytoplasm, along neurotubuli, in mitochondria and in the nucleus by use of immunoelectron microscopy. Most neuronal nitric oxide synthase (nNOS)-positive neurons were found to co-localize Cygb, although not all nNOS neurones contain Cygb. Ngb co-localize with almost all orexin neurons in the LH. In conclusion the distribution of Cygb and Ngb seems much more restricted and coherent than previously reported. We believe other functions than pure oxygen buffers and neuroprotectants should be considered. The anatomical data indicate a role in NO signalling for Cygb and involvement in sleep-wake cycling for Cygb and Ngb. (C) 2010 Elsevier B.V. All rights reserved.