BIOLOGICAL DEFENSE MECHANISMS - PRODUCTION BY LEUKOCYTES OF SUPEROXIDE A POTENTIAL BACTERICIDAL AGENT
BIOLOGICAL DEFENSE MECHANISMS - PRODUCTION BY LEUKOCYTES OF SUPEROXIDE A POTENTIAL BACTERICIDAL AGENT
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DOI：
10.1172/jci107236
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发表时间：
1973-01-01
影响因子：
15.9
通讯作者：
CURNUTTE, JT
中科院分区：
文献类型：
--
作者：
BABIOR, BM;KIPNES, RS;CURNUTTE, JT
超氧阴离子（O2-）是生物体内由氧的单电子还原产生的一种高活性物质，有可能作为白细胞的杀菌剂。当细胞色素与白细胞孵育时，发现细胞色素的还原（O2-可能作为电子供体的过程）发生。通过证明超氧化物歧化酶（一种破坏O2-的酶）可以消除白细胞介导的细胞色素减少反应，而煮沸的歧化酶、白蛋白或过氧化氢酶则不能消除该反应，O2-被确定为负责白细胞介导的细胞色素减少的因子。在这些颗粒的存在下，O2-形成的平均速率为1.03 nmol/107个细胞/15 min。然而，这个速率只是O2-产生的真实速率的下限，因为任何与细胞色素以外的成分反应的O2-都不会被检测到。因此。O2-是由白细胞在某些情况下产生的，这表明它可能参与了细菌的杀灭。
As a highly reactive substance produced in biological systems by the one-electron reduction of oxygen, superoxide (O2-) seemed a likely candidate as a bactericidal agent in leukocytes. The reduction of cytochromec, a process in which O2-may serve as an electron donor, was found to occur when the cytochrome was incubated with leukocytes. O2-was identified as the agent responsible for the leukocyte-mediated reduction of cytochromecby the demonstration that the reaction was abolished by superoxide dismutase, an enzyme that destroys O2-, but not by boiled dismutase, albumin, or catalase.Leukocyte O2-production doubled in the presence of latex particles. The average rate of formation of O2-in the presence of these particles was 1.03 nmol/107cells per 15 min. This rate, however, is only a lower limit of the true rate of O2-production, since any O2-which reacted with constituents other than cytochromecwould have gone undetected. Thus. O2-is made by leukocytes under circumstances which suggest that it may be involved in bacterial killing.