Liquid chromatographic separation and indirect detection of organic acids using iron(II) 1,10-phenanthroline as a mobile-phase additive.
Liquid chromatographic separation and indirect detection of organic acids using iron(II) 1,10-phenanthroline as a mobile-phase additive.
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使用铁(II) 1,10-菲咯啉作为流动相添加剂对有机酸进行液相色谱分离和间接检测。
DOI：
10.1021/ac00137a004
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发表时间：
1987
影响因子：
7.4
通讯作者：
Pietrzyk,DJ
中科院分区：
文献类型：
--
作者：
Rigas,PG;Pietrzyk,DJ
铁（II）1，10-菲咯啉[Fe（phen）32+]盐被用作流动相添加剂，用于在反相固定相上作为阴离子分析物的有机酸的液相色谱分离。影响选择性和分辨率的主要洗脱变量是Fe（phen）32+浓度及其在固定相上的保留、抗衡阴离子的类型和浓度、pH、缓冲液组分和浓度、离子强度、有机改性剂和分析物阴离子选择性。间接检测法是通过在510 nm处监测流出液来检测非吸收性有机分析物阴离子，Fe-（phen）~（32+）在510 nm处有最大吸收。本文给出了烷基磺酸、单-和二-烷基羧酸和芳族羧酸的多组分混合物的分离。检测限，取决于分析物和条件，ap-proach 20 ng。在20 ng至10000 ng以上的进样分析物浓度范围内，发现分析物阴离子（丙酸根和乳酸根）的检测器响应呈线性。
Iron (II) 1, 10-phenanthroline [Fe (phen) 32+] salts are used as mobile-phase additives for the liquid chromatographic separation of organic acids as anionic analytes on a reverse stationary phase. Major elution variables that affect selectivity and resolution are Fe (phen) 32+ concentration and Its retention on the stationary phase, type and concentration of counteranions, pH, buffer components and concentration, Ionic strength, organic modifier, and analyte anion selectivity. Indirect detection Is used to detect nonabsorbing organic ana-lyte anions by monitoring the effluent at 510 nm where Fe-(phen) 32+ exhibits maximum absorbance. Separations of multicomponent mixtures of alkylsuHonlc, mono-and dlprotlc alkyl carboxylic, and aromatic carboxylic acids are shown. Detection limits, depending on analyte and conditions, ap-proach 20ng. A linear detector response was found for an-alyte anion (propionate and lactate) concentrations from 20 ng to over 10000 ng of Injected analyte.