Self-referencing optrode technology for non-invasive real-time measurement of biophysical flux and physiological sensing
Self-referencing optrode technology for non-invasive real-time measurement of biophysical flux and physiological sensing
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DOI：
10.1039/b903092a
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发表时间：
2009-01-01
期刊：
影响因子：
4.2
通讯作者：
Porterfield, D. M.
中科院分区：
文献类型：
--
作者：
Chatni, M. R.;Porterfield, D. M.
振动探针技术使科学研究成为可能，扩大了我们对生物学形式和功能的了解，但细胞组学和生理组学新领域的出现将需要新技术来探测活细胞的功能领域。在本文中，我们介绍了自参考光极的开发，它代表了基于细胞和组织生理学相敏检测的下一代生物物理通量传感器。光学方法的一个关键优势是，不会受到固有电伪影的影响，而电伪影会限制传统振动或自参考探针技术的性能。在自参考模式中，光极在浓度梯度中相距几微米的两点之间振荡（0.1 Hz），根据菲克定律将光极氧浓度传感器转换为动态通量传感器。由于与相敏检测相关的固有噪声和漂移滤波，现在可以使用微光极测量皮摩尔通量水平。在本文中，我们展示了用于测量生物物理氧通量的自参考氧光极的校准、表征和应用。
Vibrating probe technology has enabled scientific investigations that have expanded our knowledge of form and function in biology, but the emergence of new fields of cytomics and physiomics will require new technologies to probe the functional realm of living cells. In this paper, we present the development of a self-referencing optrode, which represents the next generation of biophysical flux sensors based on phase-sensitive detection for cell and tissue physiology. One key advantage is that optical approaches do not suffer from the inherent electrical artifacts which limit the performance of traditional vibrating, or self-referencing probe technology. In self-referencing modality, the optrode is oscillated (0.1 Hz) between two points a few microns apart in a concentration gradient, converting the optrodic oxygen concentration sensor into a dynamic flux sensor, based on Fick's law. Because of the inherent noise and drift filtering associated with phase-sensitive detection it is now possible to measure pico-molar flux levels using a micro-optrode. In this paper, we show the calibration, characterization and application of the self-referencing oxygen optrode for measuring biophysical oxygen flux.