Roseivivax atlanticus sp. nov., isolated from surface seawater of the Atlantic Ocean
Roseivivax atlanticus sp. nov., isolated from surface seawater of the Atlantic Ocean
复制标题
DOI：
10.1007/s10482-014-0140-5
复制
发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
Guizhen Li;Qiliang Lai;Xiupian Liu;Fengqin Sun;Z. Shao
中科院分区：
文献类型：
--
作者：
Guizhen Li;Qiliang Lai;Xiupian Liu;Fengqin Sun;Z. Shao
对分离自大西洋表层海水的菌株22II-S10sT进行了分类研究。革兰氏阴性，氧化酶和过氧化氢酶阳性，杆状，以亚极性鞭毛运动。该分离物能水解明胶，但不能将硝酸盐还原为亚硝酸盐，也不能降解吐温80或七叶皂苷。在0.5 - 18%的盐度（最适值，2 - 12%）、3-10的pH（最适值，7）和10-41°C的温度（最适值，28°C）下观察到生长。基于16S rRNA基因序列的系统发育分析表明，菌株22ii - s10st1属roseivivax，序列相似性与oseivivax haloduransJCM 10272T最高（97.2%），其次为roseivivax isoporaeLMG 25204T(97.0%)；其他属的序列相似性为95.2 ~ 96.7%。菌株22II-S10sTand与两型菌株（R. haloduransJCM - 10272TandR）的DNA-DNA杂交估计值。isoporaelmg25204t)分别为22.00%和21.40%。主要脂肪酸为：总特征8 (C18:1ω7c/ω6c)（67.4%）、C18:0（7.2%）、c19:0环ω8c（7.1%）、c18:1ω7c11-甲基（6.8%）和C16:0（5.9%）。测定呼吸醌为Q-10（100%）。磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、一种氨基脂、一种糖脂和三种磷脂存在。测定染色体DNA中G+C含量为67.5 mol%。综合基因型和表型数据表明，菌株22ii - s10代表了玫瑰间日疟原虫属中的一个新种，其名称为大西洋玫瑰间日疟原虫。11，型应变22II-S10sT（= MCCC 1A09150T= LMG 27156T）。
A taxonomic study was carried out on strain 22II-S10sT, which was isolated from the surface seawater of the Atlantic Ocean. The bacterium was found to be Gram-negative, oxidase and catalase positive, rod shaped and motile by subpolar flagella. The isolate was capable of gelatine hydrolysis but unable to reduce nitrate to nitrite or degrade Tween 80 or aesculin. Growth was observed at salinities of 0.5–18 % (optimum, 2–12 %), at pH of 3–10 (optimum, 7) and at temperatures of 10–41 °C (optimum 28 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 22II-S10sTbelongs to the genusRoseivivax, with highest sequence similarity toRoseivivax haloduransJCM 10272T(97.2 %), followed byRoseivivax isoporaeLMG 25204T(97.0 %); other species of genusRoseivivaxshared 95.2–96.7 % sequence similarity. The DNA–DNA hybridization estimate values between strain 22II-S10sTand the two type strains (R. haloduransJCM 10272TandR. isoporaeLMG 25204T) were 22.00 and 21.40 %. The principal fatty acids were identified as Summed Feature 8 (C18:1ω7c/ω6c) (67.4 %), C18:0(7.2 %), C19:0cycloω8c(7.1 %), C18:1ω7c11-methyl (6.8 %) and C16:0(5.9 %). The respiratory quinone was determined to be Q-10 (100 %). Phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an aminolipid, a glycolipid and three phospholipids were present. The G+C content of the chromosomal DNA was determined to be 67.5 mol%. The combined genotypic and phenotypic data show that strain 22II-S10sTrepresents a novel species within the genusRoseivivax, for which the nameRoseivivax atlanticussp. nov. is proposed, with the type strain 22II-S10sT(= MCCC 1A09150T= LMG 27156T).