Applying microdroplets as sensors for label-free detection of chemical reactions
Applying microdroplets as sensors for label-free detection of chemical reactions
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DOI：
10.1039/c2lc20971c
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Boehm, Christian H. J.
中科院分区：
文献类型：
--
作者：
Hofmann, Tobias W.;Anselmann, Siegfried H.;Boehm, Christian H. J.
尽管其具有巨大的高通量筛选能力，但基于液滴的微流体的广泛应用仍然受到适当分析测定的不良可用性的限制。在这里，我们报告了一种新的传感器方法，该方法利用微滴大小的变化作为液滴内部反应的定量和无标记标记。我们提出了一个分析的基本机制，并应用该方法在单细胞水平上监测代谢活动。
Despite its tremendous high-throughput screening capabilities, widespread applications of droplet-based microfluidics are still limited by the poor availability of appropriate analytical assays. Here we report on a novel sensor method that exploits the osmosis-driven change in droplet size as a quantitative and label-free marker for reactions inside the droplets. We present an analysis of the underlying mechanism and apply the method for monitoring metabolic activity at a single-cell level.