Genome-wide screening differential long non-coding RNAs expression profiles discloses its roles involved in OHSS development
Genome-wide screening differential long non-coding RNAs expression profiles discloses its roles involved in OHSS development
复制标题
全基因组筛选差异长非编码 RNA 表达谱揭示了其在 OHSS 发展中的作用
DOI：
10.1007/s10815-018-1199-0
复制
发表时间：
2018-08-01
影响因子：
3.1
通讯作者：
Zhang, Qingxue
中科院分区：
文献类型：
--
作者：
Lin, Haiyan;Li, Yu;Zhang, Qingxue
目的筛选与OHSS相关的差异表达的lncRNA。OHSS被定义为卵巢过度刺激综合征。设计病例对照研究。设置大学附属医院。纳入S患者(n= 30)和低风险患者(n= 30)。干预(S)无。高危和低危患者(S)的lncRNA用于高通量测序。结果(S)共检测到23,815个LncRNA，其中4 82个差异表达(折叠变化≥2；p<； 0.0 5，FDR值<； 0.001)，其中2 0 5个上调，2 77个下调。LNC-SEC16B.1-6、LNC-SNURF-13、LNC-LGR6-6和LNC-H_2AFY2-2在颗粒细胞中表达上调，而LNC-BRD2-2、LNC-HSPA6-2和LNC-CLIC6-5在颗粒细胞中表达显著下调。这些结果得到了qRT-PCR的证实。KEGG途径和基因本体论富集化分析表明，几个生物学过程显著相关。同时，根据Cerna网络模型建立了LncRNA/miRNA相互作用网络。结论S的综合表达筛选发现了8个与OHSS过程危险因素相关的新的LncRNA。虽然目前还不清楚这些改变的lncRNAs如何调控OHSS的过程，但我们的发现表明，这些lncRNAs可能是OHSS发展过程中的新角色。
ObjectiveTo screen differentially expressed lncRNAs involved in OHSS. OHSS is defined as ovarian hyperstimulation syndrome. It is characterized as enlarged ovary and increased vascular permeability.DesignCase-control study.SettingUniversity-affiliated hospital.Patient(s)Patients with OHSS high risk (n= 30) and low risk (n= 30) were included in this study.Intervention(s)None.Main outcome measure(s)LncRNAs from women with OHSS high risk and low risk were used for high-throughput sequencing profiling. The eight most differentially expressed lncRNAs in granulosa cells were validated by semi-quantitative reverse transcription-polymerase chain reaction analysis.Result(s)A total of 23,815 lncRNAs were detected and 482 were differentially expressed (fold-change ≥2;p< 0.05, FDR value < 0.001), of which 205 were upregulated and 277 were downregulated. Lnc-SEC16B.1-6, lnc-SNURF-13, lnc-LGR6-6, and lnc-H2AFY2-2 were up-regulated, while lnc-BRD2-2, lnc-HSPA6-2, and lnc-CLIC6-5 were downregulated significantly in granulosa cells. These results were confirmed by qRT-PCR. KEGG pathways and Gene Ontology enrichment analysis revealed that several biological processes were significantly associated. Meanwhile, the lncRNA/miRNA interaction network was established according to ceRNA network model.Conclusion(s)Comprehensive expression screening identified eight novel lncRNAs associated with risk factors of OHSS process. Although it is unclear how these altered lncRNAs regulate the process of OHSS, our findings suggest these lncRNAs may be novel players in OHSS development.