Effect of d-allose on prostate cancer cell lines: phospholipid profiling by nanoflow liquid chromatography-tandem mass spectrometry
Effect of d-allose on prostate cancer cell lines: phospholipid profiling by nanoflow liquid chromatography-tandem mass spectrometry
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DOI：
10.1007/s00216-011-5113-1
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发表时间：
2011-08-01
影响因子：
4.3
通讯作者：
Moon, Myeong Hee
中科院分区：
文献类型：
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作者：
Jeong, Rae Ung;Lim, Sangsoo;Moon, Myeong Hee
d-阿洛糖是一种罕见的天然单糖，已知对癌细胞具有抗增殖作用。采用鸟枪脂质组学方法，通过磷脂（PL）分析，在分子水平上研究了d-阿洛糖对前列腺癌细胞系DU 145、前列腺癌细胞系LNCaP和正常前列腺上皮细胞（PrEC）细胞膜的影响。通过数据依赖碰撞诱导解离纳米流液相色谱-串联质谱法鉴定了85种PL物质的分子结构，包括23种磷脂酰胆碱、12种磷脂酰乙醇胺（PE）、11种磷脂酰丝氨酸（PS）、16种磷脂酰肌醇、9种磷脂酸（PA）和14种磷脂酰甘油（PG），并对PL量进行了定量。在前列腺癌细胞系的生长阶段，除了d-阿洛糖PL物种的相对调节的影响可以忽略不计或减少，但出现了几个新的PS分子（两个DU 145和三个LNCaP）。相比之下，PrEC细胞系的实验显示，一些高丰度物质（14：0/14：0-PE、16：2/16：0-PG和20：6/18：1-PA）显示浓度显著增加。这些发现支持了d-阿洛糖对前列腺癌细胞系的抗增殖作用的机制，该机制涉及诱导程序性细胞死亡，因为已知PS分子诱导细胞凋亡。主成分分析进行检查PL分布之间的差异，由d-阿洛糖促进的三种细胞系。
d-Allose, a rare, naturally occurring monosaccharide, is known to exert anti-proliferative effects on cancer cells. The effects of d-allose on the cellular membranes of hormone-refractory prostate cancer cell line (DU145), hormone-sensitive prostate cancer cell line (LNCaP), and normal prostate epithelial cells (PrEC) were studied at the molecular level by phospholipid (PL) profiling using a shotgun lipidomic method. The molecular structures of 85 PL species including 23 phosphatidylcholines, 12 phosphatidylethanolamines (PEs), 11 phosphatidylserines (PSs), 16 phosphatidylinositols, 9 phosphatidic acids (PAs), and 14 phosphatidylglycerols (PGs) were identified by data-dependent collision-induced dissociation of nanoflow liquid chromatography-tandem mass spectrometry, and the PL amounts were quantified. The addition of d-allose to prostate cancer cell lines during their growth phases had negligible or decreased effects on the relative regulation of PL species, but several new PS molecules (two for DU145 and three for LNCaP) emerged. In contrast, experiments on the PrEC cell line revealed that some high abundant species (14:0/14:0-PE, 16:2/16:0-PG, and 20:6/18:1-PA) showed significant increases in concentration. These findings support a mechanism for the anti-proliferative effect of d-allose on prostate cancer cell lines that involves the induction of programmed cell death since PS molecules are known to induce apoptosis. Principal component analysis was carried out to examine differences in PL distributions among the three cell lines promoted by d-allose.