Amino-terminal sequence of the Tn3 transposase protein.
Amino-terminal sequence of the Tn3 transposase protein.
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Tn3 转座酶蛋白的氨基末端序列。
DOI：
10.1128/jb.149.1.407-410.1982
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发表时间：
1982
影响因子：
3.2
通讯作者：
Casadaban,MJ
中科院分区：
文献类型：
--
作者：
Ditto,MD;Chou,J;Hunkapiller,MW;Fennewald,MA;Gerrard,SP;Hood,LE;Cohen,SN;Casadaban,MJ
Tn 3转座酶的氨基酸序列为Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser。这是从纯化自转座酶过量产生突变株的活性转座酶蛋白和杂合转座酶-β-半乳糖苷酶融合蛋白确定的。氨基酸序列对应于从ATG起始密码子开始的转座酶基因的DNA序列，如先前从转座酶-β-半乳糖苷酶基因融合体的分析预测的。
The amino-terminal sequence of the Tn3 transposase protein was determined to be Pro-Val-Asp-Phe-Leu-Thr-Thr-Glu-Gln-Val-Glu-Ser.... This was determined both from an active transposase protein purified from a transposase overproducing mutant strain and from a hybrid transposase-beta-galactosidase fusion protein. The amino acid sequence corresponded to the DNA sequence of the transposase gene beginning at an ATG initiation codon, as previously predicted from the analysis of transposase-beta-galactosidase gene fusions.