Simple latex agglutination assay for rapid serodiagnosis of human leptospirosis
Simple latex agglutination assay for rapid serodiagnosis of human leptospirosis
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DOI：
10.1128/jcm.38.3.1272-1275.2000
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发表时间：
2000-03-01
影响因子：
9.4
通讯作者：
Hartskeerl, RA
中科院分区：
文献类型：
--
作者：
Smits, HL;van der Hoorn, MAWG;Hartskeerl, RA
本文对一种新建立的检测人血清中钩端螺旋体属特异性抗体的乳胶凝集试验进行了评价。通过在凝集卡上将血清与等体积的稳定抗原包被、染色的供试品和对照品乳胶珠混合进行检测，并在2 min内读取结果。采用来自夏威夷、塞舌尔、泰国和荷兰的钩端螺旋体病患者和对照患者的血清样本组评价乳胶凝集试验。平均总体敏感性为82.3%，平均总体特异性为94.6%。该测定易于进行，并且不需要特殊的技能或设备。这些试剂即使在热带温度下也有很长的保质期。总之，这些因素使得该测定法甚至适合在卫生保健系统的外围水平上用作钩端螺旋体病的快速筛查试验。
A newly developed latex agglutination assay for the detection of genus-specific Leptospira antibodies in human sera was evaluated. The assay is performed by mixing, on an agglutination card, serum with equal volumes of stabilized antigen-coated, dyed test and control latex beads and is read within 2 min. The latex agglutination test was evaluated with groups of serum samples from patients with leptospirosis and control patients from Hawaii, the Seychelles, Thailand, and The Netherlands. The mean overall sensitivity was 82.3%, and the mean overall specificity was 94.6%. The assay is easy to perform and does not require special skills or equipment. The reagents have a long shelf life, even at tropical temperatures. Together, these factors make the assay suitable for use even at the peripheral level of a health care system as a rapid screening test for leptospirosis.