Expressed sequence tags from the halophyte Limonium sinense
Expressed sequence tags from the halophyte Limonium sinense
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DOI：
10.1080/10425170601108555
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发表时间：
2007-02-01
期刊：
影响因子：
--
通讯作者：
Zhang, Hui
中科院分区：
文献类型：
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作者：
Chen, Shi-Hua;Guo, Shan Li;Zhang, Hui
盐生植物能在高盐条件下生长。与糖植物相似，它们的耐盐性具有高度的遗传复杂性。关于盐生植物的形态学和生理学研究很多，但在分子水平上对盐生植物为什么具有耐盐性的研究却很少。中华补血草是一种泌盐型盐生植物，可通过多细胞腺体排盐。本文报道了一个以叶组织为材料的苜蓿cDNA文库的构建和序列分析。sinenes。在获得的1082个表达序列标签（EST）中，鉴定了684个独特的基因：429个与先前鉴定的基因同源，255个与未鉴定的基因匹配。与其他EST数据库相比，该数据库具有盐生植物特有的特征，如与细胞骨架、细胞内运输、细胞膜转运相关的基因丰度。
Halophytes can grow under a high salinity condition. Similar to glycophytes, their salt-tolerance possesses a high genetic complexity. There are many morphological and physiological studies on halophytes but very little information is at molecular level why they are salt-tolerant. Limonium sinense is a salt-secreting halophyte and can excretes salts by multi-cellular glands. Here, we report the library construction and sequence analysis of a cDNA library made from leaf tissue of L. sinenes. Among those 1082 expressed sequence tag (EST) obtained, 684 unique genes were identified: 429 showed homology to previously identified genes, 255 matched to uncharacterized genes. Compared with other EST databases, some characteristic features such as abundance genes in related to cytoskeleton and intracellular traffic, membrane transporting were observed, which may be specific to halophytes.