Prenatal development of mouse central nervous structures: time of neuron origin and gradients of neuronal production. A radioautographic study.
Prenatal development of mouse central nervous structures: time of neuron origin and gradients of neuronal production. A radioautographic study.
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小鼠中枢神经结构的产前发育：神经元起源时间和神经元产生梯度。
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发表时间：
1982
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通讯作者：
Bisconte Jc
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作者：
R. Gardette;M. Courtois;Bisconte Jc
为了获得小鼠主要中枢神经结构中神经发生的总体视图，我们对神经元起源的时间和神经元产生的梯度进行了系统的研究。这些动物是在受孕后选定日期给予3 H-胸苷脉冲标记的妊娠雌性动物的后代。这些日子被选择来涵盖主要的大脑发育阶段：E10.5，E11.5，E12.5，E13.5，E14.5，E15.5，E16.5，E17.5和E18.5。在出生后第4周处死后代，计算不同脑结构的标记指数。作为一个函数的注射和研究的大脑水平的标记指数的研究为我们提供了在这些结构中的神经元增殖的主要时期的信息，并使我们能够分析给定的神经结构内的形成梯度。作为一个整体，对于大多数神经结构（嗅觉，皮层，隔，海马，视前区，丘脑，上丘脑，下丘脑和小脑结构），我们的结果是在良好的协议与已经发表的意见。然而，我们概述了两个主要的现象，显然是未观察到的，直到现在在小鼠：第一个是在下丘的神经元生产的rostrocaudal梯度，第二个是长期的神经元增殖的尾状核持续从E11.5到E18.5，沿着一个复杂的内-外和mediolateral梯度。
In order to obtain an overall view of neurogenesis in the main central nervous structures of the mouse, we have undertaken a systematic study of the time of neuron origin and of the gradients of neuronal production. The animals were the offspring of pregnant females given a pulse label of 3H-thymidine on selected days after conception. These days were chosen to cover the principal brain development stages: E10.5, E11.5, E12.5, E13.5, E14.5, E15.5, E16.5, E17.5 and E18.5. The offspring were sacrificed during the fourth postnatal week and labeling indices were calculated in the different brain structures. The study of labeling indices as a function of the day of injection and of the brain level studied provided us with information on the major periods of neuronal proliferation in these structures and allowed us to analyse the formation gradients inside a given nervous structure. As a whole, for a majority of nervous structures (olfactory, cortical, septal, hippocampal, preoptic, thalamic, epithalamic, hypothalamic and cerebellar structures), our results are in good agreement with the already published observations. However, we outlined two major phenomena apparently unobserved until now in the mouse: the first is a rostrocaudal gradient of neuronal production in the inferior colliculi, and the second is the long period of neuronal proliferation in the caudate nucleus lasting from E11.5 to E18.5, along a complex inside-out and mediolateral gradient.