EFFECT OF ANTIOXIDANTS ON CULTURED HUMAN DIPLOID FIBROBLASTS EXPOSED TO CYSTINE-FREE MEDIUM
EFFECT OF ANTIOXIDANTS ON CULTURED HUMAN DIPLOID FIBROBLASTS EXPOSED TO CYSTINE-FREE MEDIUM
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DOI：
10.1016/0006-291x(77)90623-4
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发表时间：
1977-01-01
影响因子：
3.1
通讯作者：
OKUMURA, H
中科院分区：
文献类型：
--
作者：
BANNAI, S;TSUKEDA, H;OKUMURA, H
对数生长的人胚二倍体细胞在无胱氨酸培养基中18h内开始死亡。细胞内的酸溶性巯基化合物几乎全部由谷胱甘肽构成，胱氨酸耗竭后细胞内谷胱甘肽水平迅速下降。通过添加维生素E，细胞在无胱氨酸的培养基中存活超过6天，尽管细胞的谷胱甘肽含量降低到低于正常水平的1%。合成抗氧化剂也有类似的效果，并提出了细胞在无胱氨酸培养基中死亡的机制。
Logarithmically growing human embryonic diploid cells started to die in cystine-free medium within 18 h. Glutathione accounted for almost all the acid-soluble sulfhydryl compound of the cells and cellular glutathione level decreased rapidly after cystine depletion. By adding vitamin E the cells survived over 6 days in cystine-free medium, though glutathione content of the cells was reduced to less than 1% of the normal level. Synthetic antioxidants had a similar effect, and a mechanism by which cells die in cystine-free medium was suggested.