Opportunities for CRISPR/Cas9 Gene Editing in Retinal Regeneration Research.
Opportunities for CRISPR/Cas9 Gene Editing in Retinal Regeneration Research.
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DOI：
10.3389/fcell.2017.00099
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发表时间：
2017
影响因子：
5.5
通讯作者：
Hyde DR
中科院分区：
文献类型：
--
作者：
Campbell LJ;Hyde DR
虽然视网膜变性和疾病会导致人类永久性损伤和视力丧失，但严重受损的斑马鱼视网膜具有很高的再生失去的神经元和恢复视觉行为的能力。在理解这种再生反应的分子和细胞基础方面的进展给人们带来了希望，可以开发出策略和治疗方法来恢复盲人和视力受损者的视力。斑马鱼对分子工具、成像技术以及正向和反向遗传方法的顺从性促进了我们目前的理解。因此，斑马鱼研究界已经开发了用于发育和成年动物的各种研究工具，包括用于促进转基因动物的产生的工具包，用于诱导的细胞特异性转基因表达的系统，以及几乎每个蛋白质编码基因的敲除等位基因的产生。随着CRISPR/Cas9基因组编辑已经开始彻底改变分子生物学研究，斑马鱼社区已经通过开发针对斑马鱼的CRISPR/Cas9技术以及将CRISPR/Cas9整合到可用的工具集中做出了积极的回应。CRISPR/Cas9在视网膜再生研究中的应用无疑将使我们更接近于了解斑马鱼视网膜修复和视力恢复的机制，以及开发恢复盲人和视力受损个体视力的治疗方法。本文重点介绍CRISPR/Cas9如何整合到斑马鱼研究工具中，以及这种新工具将如何彻底改变视网膜再生研究领域。
While retinal degeneration and disease results in permanent damage and vision loss in humans, the severely damaged zebrafish retina has a high capacity to regenerate lost neurons and restore visual behaviors. Advancements in understanding the molecular and cellular basis of this regeneration response give hope that strategies and therapeutics may be developed to restore sight to blind and visually-impaired individuals. Our current understanding has been facilitated by the amenability of zebrafish to molecular tools, imaging techniques, and forward and reverse genetic approaches. Accordingly, the zebrafish research community has developed a diverse array of research tools for use in developing and adult animals, including toolkits for facilitating the generation of transgenic animals, systems for inducible, cell-specific transgene expression, and the creation of knockout alleles for nearly every protein coding gene. As CRISPR/Cas9 genome editing has begun to revolutionize molecular biology research, the zebrafish community has responded in stride by developing CRISPR/Cas9 techniques for the zebrafish as well as incorporating CRISPR/Cas9 into available toolsets. The application of CRISPR/Cas9 to retinal regeneration research will undoubtedly bring us closer to understanding the mechanisms underlying retinal repair and vision restoration in the zebrafish, as well as developing therapeutic approaches that will restore vision to blind and visually-impaired individuals. This review focuses on how CRISPR/Cas9 has been integrated into zebrafish research toolsets and how this new tool will revolutionize the field of retinal regeneration research.
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影响因子：
8
作者：
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通讯作者：
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影响因子：
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Hyde DR
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