Development of an optical fiber SPR sensor for living cell activation
Development of an optical fiber SPR sensor for living cell activation
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DOI：
10.1016/j.bios.2009.09.042
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发表时间：
2010-01-15
影响因子：
12.6
通讯作者：
Hide, Michihiro
中科院分区：
文献类型：
--
作者：
Yanase, Yuhki;Araki, Atsunori;Hide, Michihiro
表面等离子体共振（SPR）传感器为研究生物分子的相互作用和活细胞在传感器芯片上的反应提供了一种有用的手段。然而，传统的SPR传感器体积庞大。作为普通诊断设备，使用起来既昂贵又复杂。在本研究中，我们开发了一个相对小而简单的SPR系统。使用直径为250pm的光纤来检测附着在光纤尖端的活细胞的激活情况。该系统在直径为200pm、长度为1cm的光纤芯上涂覆厚度为50nm的金膜，引起等离子体共振。由白光LED发出的光由于传感器部分的SPR现象而衰减，使用光谱检测器进行检测和分析。具有不同折射率的溶剂和与传感器尖端的蛋白质结合的差异具有足够的灵敏度。此外，当RBL-2H3肥大细胞固定在纤维尖端并受到抗原刺激时，实时检测到AR持续增加。这种小纤维SPR系统可以作为各种体内或体外临床检查的有用工具。(C) 2009 Elsevier B.V.版权所有
Surface plasmon resonance (SPR) sensors provide a useful means to study the interactions of biological molecules and the reaction of living cells on a sensor chip. However, conventional SPR sensors are bulky. expensive and complicated to use as common diagnostic equipment. In this study, we developed a relatively small and simple SPR system. using optical fibers of 250 pm diameter to detect the activation of living cells attached to the fiber tip. For this system, the core of 200 pm diameter with I cm length of an optical fiber was coated by gold film with 50 nm thickness to cause plasmon resonance. The light provided by a white LED and attenuated due to a SPR phenomenon in the sensor part was detected and analyzed using a spectrum detector. The difference in solvents with various refractive indexes and protein-bindings to the sensor tip was detected with sufficient sensitivity. Moreover, it detected a sustained increase of AR in a real-time manner, when RBL-2H3 mast cells were fixed onto the fiber tip and stimulated by an antigen. This small fiber SPR system might serve as a useful toot for various clinical examinations either within or outside the body. (C) 2009 Elsevier B.V. All rights reserved.